DOI: 10.1016/j.atmosenv.2018.02.025
Scopus记录号: 2-s2.0-85042178616
论文题名: Assessing the cytotoxicity of ambient particulate matter (PM) using Chinese hamster ovary (CHO) cells and its relationship with the PM chemical composition and oxidative potential
作者: Wang Y ; , Plewa M ; J ; , Mukherjee U ; K ; , Verma V
刊名: Atmospheric Environment
ISSN: 0168-2563
EISSN: 1573-515X
出版年: 2018
卷: 179 起始页码: 132
结束页码: 141
语种: 英语
英文关键词: Chinese hamster ovary (CHO) cells
; Cytotoxicity
; Dithiothreitol
; Reactive oxygen species (ROS) generation
; Surrogate lung fluid
Scopus关键词: Assays
; Biological organs
; Cells
; Copper
; Cytology
; Cytotoxicity
; Lead
; Mammals
; Manganese
; Manganese removal (water treatment)
; Organic carbon
; Particulate emissions
; Urban growth
; Ambient particulate Matter
; Chemical compositions
; Chinese Hamster ovary cells
; Dithiothreitol
; Reactive oxygen species
; Surrogate lungs
; Synergistic interaction
; Water-soluble organic carbon
; Iron compounds
; chromium
; cobalt
; cupric ion
; dithiothreitol
; ferric ion
; hydroxyl radical
; lead
; manganese
; mercury
; nickel
; organic carbon
; reactive oxygen metabolite
; zinc
; ambient air
; animal cell
; Article
; cell assay
; chemical composition
; CHO cell line
; concentration response
; cytotoxicity
; cytotoxicity assay
; female
; LC50
; nonhuman
; oxidation reduction potential
; particulate matter
; priority journal
; seasonal variation
; spring
; summer
; total organic carbon
; winter
; Cricetulus griseus
; Mammalia
Scopus学科分类: Environmental Science: Water Science and Technology
; Earth and Planetary Sciences: Earth-Surface Processes
; Environmental Science: Environmental Chemistry
英文摘要: We assessed mammalian cell cytotoxicity of ambient PM2.5 and investigated its association with the oxidative potential (OP) and chemical composition of the particles. Sixteen PM samples spanning in various seasons (fall, winter, spring and summer) were collected from an urban site in central Illinois. Cytotoxicity (LC50) in terms of the volume of air that kills 50% of the cells were calculated, which varied from 4.3 to 7.2 m3 of air. The OP was measured by two assays – the dithiothreitol (DTT) and the surrogate lung fluid (SLF) assay. In DTT assay, we measured two endpoints – hydroxyl radicals (•OH) generation and DTT consumption (the conventionally measured endpoint), while only •OH generation was measured in the SLF assay. Although, all three endpoints in the OP assays correlated significantly (P ≤ 0.05) with LC50, the correlation of reactive oxygen species (ROS) generation in DTT and SLF assays was much higher (r > 0.80 for •OH generation versus LC50) than the DTT consumption (r = 0.58). To further understand the components in PM that drive cytotoxicity and OP, concentration of water-soluble metals (Fe, Cu, Co, Cr, Mn, Ni, Pb, V, Hg, and Zn), organic carbon (OC), water soluble organic carbon (WSOC), and elemental carbon (EC) were measured. Among all the chemical components, Fe, Cu and WSOC correlated most (r > 0.70; P ≤ 0.01) with the cytotoxicity. DTT consumption correlated only with OC and WSOC (r > 0.80; P ≤ 0.01), while •OH generation in DTT and SLF assay correlated with both WSOC (r > 0.70; P ≤ 0.01) and metals (i.e. Fe and Cu; r > 0.75; P ≤ 0.01). Our results suggest a strong link between the PM2.5 OP and its cytotoxicity. Furthermore, the synergistic interactions among the organic compounds (i.e. WSOC) and metals (Fe and Cu) to enhance the ROS generation, which are more effectively captured in •OH generation endpoints in DTT and SLF assay than the DTT consumption, appear to be largely responsible for the observed mammalian cell cytotoxicity of PM2.5. © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/82995
Appears in Collections: 气候变化事实与影响
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作者单位: Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, United States; Department of Crop Sciences, Safe Global Water Institute, University of Illinois at Urbana-Champaign, United States; College of Business, University of Illinois at Urbana-Champaign, United States
Recommended Citation:
Wang Y,, Plewa M,J,et al. Assessing the cytotoxicity of ambient particulate matter (PM) using Chinese hamster ovary (CHO) cells and its relationship with the PM chemical composition and oxidative potential[J]. Atmospheric Environment,2018-01-01,179