DOI: 10.1007/s10533-016-0188-6
Scopus记录号: 2-s2.0-84959510549
论文题名: Alternative nitrogenase activity in the environment and nitrogen cycle implications
作者: Zhang X. ; McRose D.L. ; Darnajoux R. ; Bellenger J.P. ; Morel F.M.M. ; Kraepiel A.M.L.
刊名: Biogeochemistry
ISSN: 0168-2563
EISSN: 1573-515X
出版年: 2016
卷: 127, 期: 2018-02-03 起始页码: 189
结束页码: 198
语种: 英语
英文关键词: Alternative nitrogenase
; Nitrogen cycle
; Nitrogen fixation
; Stable isotopes
; Trace metals
英文摘要: Biological nitrogen fixation, the main natural input of fixed nitrogen into the biosphere, is catalyzed by Mo-, V-, or Fe-only nitrogenase metalloenzymes. Although “alternative” V- and Fe-only nitrogenase genes are found in many environments, the contribution of these isoenzymes to N2 fixation is unknown. Here we present a new method (ISARA, isotopic acetylene reduction assay) that distinguishes canonical Mo and alternative nitrogenase activities based on in vivo 13C fractionation of acetylene reduction to ethylene (13εMo = 13.1–14.7 ‰, 13εV = 7.5–8.8 ‰, 13εFe = 5.8–6.5 ‰). ISARA analyses indicate significant contributions of alternative nitrogen fixation in boreal cyanolichens and salt marshes (~10–40 % acetylene reduction, ~20–55 % N2 fixed). These results affect the quantitative interpretation of natural abundance 15N data or traditional acetylene reduction assays. They also invite a reexamination of the conditions under which the different nitrogenase isozymes are active and suggest significant interactions between the cycles of nitrogen and trace metals. © 2016, Springer International Publishing Switzerland. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/83411
Appears in Collections: 气候减缓与适应 气候变化事实与影响
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作者单位: Department of Geosciences, Princeton University, Guyot Hall, Princeton, NJ, United States; Department of Chemistry, Princeton University, Princeton, NJ, United States; Department of Chemistry, Sherbrooke University, Québec, Canada
Recommended Citation:
Zhang X.,McRose D.L.,Darnajoux R.,et al. Alternative nitrogenase activity in the environment and nitrogen cycle implications[J]. Biogeochemistry,2016-01-01,127(2018-02-03)