DOI: 10.1007/s10533-013-9933-2
Scopus记录号: 2-s2.0-84896397886
论文题名: Molecular assays advance understanding of sulfate reduction despite cryptic cycles
作者: Reese B.K. ; Witmer A.D. ; Moller S. ; Morse J.W. ; Mills H.J.
刊名: Biogeochemistry
ISSN: 0168-2563
EISSN: 1573-515X
出版年: 2014
卷: 118, 期: 2018-01-03 起始页码: 307
结束页码: 319
语种: 英语
英文关键词: Cryptic sulfur cycling
; dsrA quantification
; Microbial ecology
; Pyrosequencing
; Sulfate reduction rate
Scopus关键词: bioassay
; biogeochemical cycle
; carbon budget
; electron
; estuarine sediment
; marine environment
; molecular analysis
; nutrient budget
; oxidation
; radioactivity
; sediment chemistry
; sulfate
; sulfide
; sulfur cycle
; Texas
; United States
英文摘要: Abiotic and secondary biotic reactions can contribute to the formation of cryptic biogeochemical cycles, resulting in an underestimation of carbon and nutrient budgets. This Texas coastal estuary sediment study provided a unique opportunity to use multidisciplinary RNA-based molecular and geochemical approaches to identify cryptic cycles associated with sulfate reduction, a commonly measured biogeochemical process considered to be the predominant anoxic terminal electron accepting process in shallow marine environments. Active sulfate reduction within an environment is typically determined by the detection of sulfides. However, a biologically driven cryptic cycle was determined by identifying metabolically active sulfate reducing and sulfur oxidizing lineages co-locating within the sediments, effectively masking sulfide production through re-oxidation back to sulfate. Similar co-location of sulfate and iron reducing lineages prevented the detection of sulfides through the formation of iron sulfide minerals, producing a geochemically driven cryptic cycle. We also showed that sulfate reduction rates determined by 35SO4 2- incubation analysis can be positively correlated with dsrA transcript abundance, and thus this molecular technique may be a proxy for the prohibitive radioactive method. Based on these results, support is given to a synergistic geochemical and molecular biological strategy to better provide an understanding of marine sulfur cycle. © 2013 Springer Science+Business Media Dordrecht. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/83659
Appears in Collections: 气候减缓与适应 气候变化事实与影响
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作者单位: University of Southern California, Los Angeles, CA, United States; Georgia Southern University, Statesboro, GA, United States; Texas A and M University, College Station, TX, United States; University of Houston Clear Lake, Houston, TX, United States
Recommended Citation:
Reese B.K.,Witmer A.D.,Moller S.,et al. Molecular assays advance understanding of sulfate reduction despite cryptic cycles[J]. Biogeochemistry,2014-01-01,118(2018-01-03)