DOI: 10.1007/s10533-013-9898-1
Scopus记录号: 2-s2.0-84897375544
论文题名: Different temperature sensitivity and kinetics of soil enzymes indicate seasonal shifts in C, N and P nutrient stoichiometry in acid forest soil
作者: Bárta J. ; Šlajsová P. ; Tahovská K. ; Picek T. ; Šantrůčková H.
刊名: Biogeochemistry
ISSN: 0168-2563
EISSN: 1573-515X
出版年: 2014
卷: 117, 期: 2018-02-03 起始页码: 525
结束页码: 537
语种: 英语
英文关键词: Acid forest soil
; Enzyme kinetics
; Potential enzyme activity modeling
; Soil enzymes
; Temperature sensitivity
Scopus关键词: acid soil
; enzyme activity
; forest soil
; numerical model
; phosphorus
; reaction kinetics
; sensitivity analysis
; soil carbon
; soil horizon
; soil nitrogen
; soil nutrient
; soil organic matter
; soil temperature
; temperature effect
; Bohemian Forest
; Bohemian Massif
; Bacteria (microorganisms)
英文摘要: Acid forest soils in the Bohemian Forest in Central Europe are biogeochemically imbalanced in organic C, N and P processing. We hypothesized that these imbalances can be due to different temperature sensitivities of soil enzyme activities and their affinities to substrate in litter and organic soil horizons. We measured potential activities of five main soil enzymes (β-glucosidase, cellobiohydrolase, Leu-aminopeptidase, Ala-aminopeptidase, and phosphatase) responsible for organic carbon, nitrogen and phosphorus acquisition. We also modeled potential in situ enzyme activities and nutrient release based on continuous in situ temperature measurements. We determined basic kinetic parameters (Km, Vmax), enzyme efficiencies (kcat) and temperature sensitivities (Ea and Q10) according to Michaelis-Menten kinetic and modified Arrhenius models. Our results showed significant differences in substrate affinities between the litter and organic soil horizons. Higher aminopeptidase affinity (lower Km) in the litter soil horizon can lead to leaching of peptidic compounds to lower soil horizons. β-Glucosidase and phosphatase showed high temperature response following the Arrhenius model. However, both aminopeptidases showed no or even decreased activity with increasing temperature. The aminopeptidase temperature insensitivity means that peptidic compounds are degraded at the same or even lower rate in warmer and colder periods of the year in acid forest soils. This imbalance results in different release of available nutrients from plant litter and soil organic matter which may affect bacterial and fungal community composition and nutrient leaching from these ecosystems. © 2013 Springer Science+Business Media Dordrecht. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/83716
Appears in Collections: 气候减缓与适应 气候变化事实与影响
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作者单位: Department of Ecosystem Biology, University of South Bohemia, Branišovská 31, 370 05 Ceske Budejovice, Czech Republic
Recommended Citation:
Bárta J.,Šlajsová P.,Tahovská K.,et al. Different temperature sensitivity and kinetics of soil enzymes indicate seasonal shifts in C, N and P nutrient stoichiometry in acid forest soil[J]. Biogeochemistry,2014-01-01,117(2018-02-03)