globalchange  > 影响、适应和脆弱性
DOI: 10.1111/gbi.12187
Scopus记录号: 2-s2.0-84978946469
论文题名:
Biofilm adaptation to iron availability in the presence of biotite and consequences for chemical weathering
作者: Grant M.R.; Tymon L.S.; Helms G.L.; Thomashow L.S.; Kent Keller C.; Harsh J.B.
刊名: Geobiology
ISSN: 1472-4677
EISSN: 1472-4669
出版年: 2016
卷: 14, 期:6
起始页码: 588
结束页码: 598
语种: 英语
Scopus关键词: bacterium ; biofilm ; biotite ; cation ; chemical weathering ; environmental factor ; glass ; iron ; leaching ; nuclear magnetic resonance ; polysaccharide ; smoothing ; soil microorganism ; Pseudomonas putida ; aluminum silicate ; biotite ; ferrous ion ; iron ; bacterial phenomena and functions ; biofilm ; growth, development and aging ; metabolism ; microbiology ; nuclear magnetic resonance spectroscopy ; pine ; plant root ; scanning electron microscopy ; Aluminum Silicates ; Bacterial Physiological Phenomena ; Biofilms ; Ferrous Compounds ; Iron ; Magnetic Resonance Spectroscopy ; Microscopy, Electron, Scanning ; Pinus ; Plant Roots
Scopus学科分类: Earth and Planetary Sciences: General Earth and Planetary Sciences ; Environmental Science: General Environmental Science ; Agricultural and Biological Sciences: Ecology, Evolution, Behavior and Systematic
英文摘要: Bacteria in nature often live within biofilms, exopolymeric matrices that provide a favorable environment that can differ markedly from their surroundings. Biofilms have been found growing on mineral surfaces and are expected to play a role in weathering those surfaces, but a clear understanding of how environmental factors, such as trace-nutrient limitation, influence this role is lacking. Here, we examine biofilm development by Pseudomonas putida in media either deficient or sufficient in Fe during growth on biotite, an Fe rich mineral, or on glass. We hypothesized that the bacteria would respond to Fe deficiency by enhancing biotite dissolution and by the formation of binding sites to inhibit Fe leaching from the system. Glass coupons acted as a no-Fe control to investigate whether biofilm response depended on the presence of Fe in the supporting solid. Biofilms grown on biotite, as compared to glass, had significantly greater biofilm biomass, specific numbers of viable cells (SNVC), and biofilm cation concentrations of K, Mg, and Fe, and these differences were greater when Fe was deficient in the medium. Scanning electron microscopy (SEM) confirmed that biofilm growth altered the biotite surface, smoothing the rough, jagged edges of channels scratched by hand on the biotite, and dissolving away small, easy-to-access particles scattered across the planar surface. High-resolution magic angle spinning proton nuclear magnetic resonance (HRMAS 1H NMR) spectroscopy showed that, in the Fe-deficient medium, the relative amount of polysaccharide nearly doubled relative to that in biofilms grown in the medium amended with Fe. The results imply that the bacteria responded to the Fe deficiency by obtaining Fe from biotite and used the biofilm matrix to enhance weathering and as a sink for released cation nutrients. These results demonstrate one mechanism by which biofilms may help soil microbes overcome nutrient deficiencies in oligotrophic systems. © 2016 John Wiley & Sons Ltd
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/85087
Appears in Collections:影响、适应和脆弱性

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作者单位: Department of Crops and Soil Sciences, Washington State University, Pullman, WA, United States; Department of Plant Soil and Entomological Sciences, University of Idaho, Moscow, ID, United States; Nuclear Magnetic Resonance Spectroscopy Center, Washington State University, Pullman, WA, United States; Root Disease and Biological Control Research Unit, USDA-ARS, Washington State University, Pullman, WA, United States; School of the Environment, Washington State University, Pullman, WA, United States

Recommended Citation:
Grant M.R.,Tymon L.S.,Helms G.L.,et al. Biofilm adaptation to iron availability in the presence of biotite and consequences for chemical weathering[J]. Geobiology,2016-01-01,14(6)
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