DOI: 10.1111/gbi.12140
Scopus记录号: 2-s2.0-84937976530
论文题名: Metabolic associations with archaea drive shifts in hydrogen isotope fractionation in sulfate-reducing bacterial lipids in cocultures and methane seeps
作者: Dawson K.S. ; Osburn M.R. ; Sessions A.L. ; Orphan V.J.
刊名: Geobiology
ISSN: 1472-4677
EISSN: 1472-4669
出版年: 2015
卷: 13, 期: 5 起始页码: 462
结束页码: 477
语种: 英语
Scopus关键词: biomarker
; concentration (composition)
; fatty acid
; hydrogen isotope
; isotopic fractionation
; laboratory method
; lipid
; metabolism
; methane
; sediment core
; sulfate-reducing bacterium
; Bacteria (microorganisms)
; Deltaproteobacteria
; Desulfococcus multivorans
; Methanosarcina acetivorans
; deuterium
; hydrogen
; lipid
; sea water
; chemistry
; Deltaproteobacteria
; growth, development and aging
; lipid metabolism
; metabolism
; Methanosarcina
; microbial consortium
; microbiology
; Oregon
; Deltaproteobacteria
; Deuterium
; Hydrogen
; Lipid Metabolism
; Lipids
; Methanosarcina
; Microbial Consortia
; Oregon
; Seawater
Scopus学科分类: Earth and Planetary Sciences: General Earth and Planetary Sciences
; Environmental Science: General Environmental Science
; Agricultural and Biological Sciences: Ecology, Evolution, Behavior and Systematic
英文摘要: Correlation between hydrogen isotope fractionation in fatty acids and carbon metabolism in pure cultures of bacteria indicates the potential of biomarker D/H analysis as a tool for diagnosing carbon substrate usage in environmental samples. However, most environments, in particular anaerobic habitats, are built from metabolic networks of micro-organisms rather than a single organism. The effect of these networks on D/H of lipids has not been explored and may complicate the interpretation of these analyses. Syntrophy represents an extreme example of metabolic interdependence. Here, we analyzed the effect of metabolic interactions on the D/H biosignatures of sulfate-reducing bacteria (SRB) using both laboratory maintained cocultures of the methanogen Methanosarcina acetivorans and the SRB Desulfococcus multivorans in addition to environmental samples harboring uncultured syntrophic consortia of anaerobic methane-oxidizing archaea (ANME) and sulfate-reducing Deltaproteobacteria (SRB) recovered from deep-sea methane seeps. Consistent with previously reported trends, we observed a ~80‰ range in hydrogen isotope fractionation (εlipid-water ) for D. multivorans grown under different carbon assimilation conditions, with more D-enriched values associated with heterotrophic growth. In contrast, for cocultures of D. multivorans with M. acetivorans, we observed a reduced range of εlipid - water values (~36‰) across substrates with shifts of up to 61‰ compared to monocultures. Sediment cores from methane seep settings in Hydrate Ridge (offshore Oregon, USA) showed similar D-enrichment in diagnostic SRB fatty acids coinciding with peaks in ANME/SRB consortia concentration suggesting that metabolic associations are connected to the observed shifts in εlipid-water values. © 2015 John Wiley & Sons Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/85134
Appears in Collections: 影响、适应和脆弱性
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作者单位: Division of Geological and Planetary Science, California Institute of Technology, Pasadena, CA, United States; Penn State Astrobiology Research Center, Pennsylvania State University, University Park, PA, United States; Department of Earth and Planetary Sciences, Northwestern University, Evanston, IL, United States
Recommended Citation:
Dawson K.S.,Osburn M.R.,Sessions A.L.,et al. Metabolic associations with archaea drive shifts in hydrogen isotope fractionation in sulfate-reducing bacterial lipids in cocultures and methane seeps[J]. Geobiology,2015-01-01,13(5)