globalchange  > 影响、适应和脆弱性
DOI: 10.1111/gbi.12088
Scopus记录号: 2-s2.0-84902303332
论文题名:
3-D analysis of bacterial cell-(iron)mineral aggregates formed during Fe(II) oxidation by the nitrate-reducing Acidovorax sp. strain BoFeN1 using complementary microscopy tomography approaches
作者: Schmid G.; Zeitvogel F.; Hao L.; Ingino P.; Floetenmeyer M.; Stierhof Y.-D.; Schroeppel B.; Burkhardt C.J.; Kappler A.; Obst M.
刊名: Geobiology
ISSN: 1472-4677
EISSN: 1472-4669
出版年: 2014
卷: 12, 期:4
起始页码: 340
结束页码: 361
语种: 英语
Scopus关键词: bacterium ; cytoplasm ; iron ; oxidation ; scanning electron microscopy ; sorption ; spatial resolution ; tomography ; transmission electron microscopy ; iron ; iron derivative ; mineral ; nitric acid derivative ; chemistry ; Comamonadaceae ; electron tomography ; metabolism ; oxidation reduction reaction ; Comamonadaceae ; Electron Microscope Tomography ; Iron ; Iron Compounds ; Minerals ; Nitrates ; Oxidation-Reduction
Scopus学科分类: Earth and Planetary Sciences: General Earth and Planetary Sciences ; Environmental Science: General Environmental Science ; Agricultural and Biological Sciences: Ecology, Evolution, Behavior and Systematic
英文摘要: The formation of cell-(iron)mineral aggregates as a consequence of bacterial iron oxidation is an environmentally widespread process with a number of implications for processes such as sorption and coprecipitation of contaminants and nutrients. Whereas the overall appearance of such aggregates is easily accessible using 2-D microscopy techniques, the 3-D and internal structure remain obscure. In this study, we examined the 3-D structure of cell-(iron)mineral aggregates formed during Fe(II) oxidation by the nitrate-reducing Acidovorax sp. strain BoFeN1 using a combination of advanced 3-D microscopy techniques. We obtained 3-D structural and chemical information on different cellular encrustation patterns at high spatial resolution (4-200 nm, depending on the method): more specifically, (1) cells free of iron minerals, (2) periplasm filled with iron minerals, (3) spike- or platelet-shaped iron mineral structures, (4) bulky structures on the cell surface, (5) extracellular iron mineral shell structures, (6) cells with iron mineral filled cytoplasm, and (7) agglomerations of extracellular globular structures. In addition to structural information, chemical nanotomography suggests a dominant role of extracellular polymeric substances (EPS) in controlling the formation of cell-(iron)mineral aggregates. Furthermore, samples in their hydrated state showed cell-(iron)mineral aggregates in pristine conditions free of preparation (i.e., drying/dehydration) artifacts. All these results were obtained using 3-D microscopy techniques such as focused ion beam (FIB)/scanning electron microscopy (SEM) tomography, transmission electron microscopy (TEM) tomography, scanning transmission (soft) X-ray microscopy (STXM) tomography, and confocal laser scanning microscopy (CLSM). It turned out that, due to the various different contrast mechanisms of the individual approaches, and due to the required sample preparation steps, only the combination of these techniques was able to provide a comprehensive understanding of structure and composition of the various Fe-precipitates and their association with bacterial cells and EPS. © 2014 John Wiley & Sons Ltd.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/85168
Appears in Collections:影响、适应和脆弱性

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作者单位: Center for Applied Geoscience, University of Tuebingen, Tuebingen, Germany; Max Planck Institute for Developmental Biology, Tuebingen, Germany; Center for Plant Molecular Biology, University of Tuebingen, Tuebingen, Germany; NMI Natural and Medical Sciences Institute, University of Tuebingen, Reutlingen, Germany

Recommended Citation:
Schmid G.,Zeitvogel F.,Hao L.,et al. 3-D analysis of bacterial cell-(iron)mineral aggregates formed during Fe(II) oxidation by the nitrate-reducing Acidovorax sp. strain BoFeN1 using complementary microscopy tomography approaches[J]. Geobiology,2014-01-01,12(4)
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