globalchange  > 影响、适应和脆弱性
DOI: 10.1111/gbi.12080
Scopus记录号: 2-s2.0-84898645770
论文题名:
Identification of Desulfobacterales as primary hydrogenotrophs in a complex microbial mat community
作者: Burow L.C.; Woebken D.; Marshall I.P.G.; Singer S.W.; Pett-Ridge J.; Prufert-Bebout L.; Spormann A.M.; Bebout B.M.; Weber P.K.; Hoehler T.M.
刊名: Geobiology
ISSN: 1472-4677
EISSN: 1472-4669
出版年: 2014
卷: 12, 期:3
起始页码: 221
结束页码: 230
语种: 英语
Scopus关键词: hydrogen ; sulfate ; transcriptome ; bacterial gene ; classification ; Deltaproteobacteria ; genetics ; isolation and purification ; metabolism ; molecular genetics ; oxidation reduction reaction ; physiology ; polymerase chain reaction ; RNA gene ; sequence analysis ; United States ; California ; Deltaproteobacteria ; Genes, Bacterial ; Genes, rRNA ; Hydrogen ; Molecular Sequence Data ; Oxidation-Reduction ; Polymerase Chain Reaction ; Sequence Analysis, Protein ; Sulfates ; Transcriptome
Scopus学科分类: Earth and Planetary Sciences: General Earth and Planetary Sciences ; Environmental Science: General Environmental Science ; Agricultural and Biological Sciences: Ecology, Evolution, Behavior and Systematic
英文摘要: Hypersaline microbial mats have been shown to produce significant quantities of H2 under dark, anoxic conditions via cyanobacterial fermentation. This flux of a widely accessible microbial substrate has potential to significantly influence the ecology of the mat, and any consumption will affect the net efflux of H2 that might otherwise be captured as a resource. Here, we focus on H2 consumption in a microbial mat from Elkhorn Slough, California, USA, for which H2 production has been previously characterized. Active biologic H2 consumption in this mat is indicated by a significant time-dependent decrease in added H2 compared with a killed control. Inhibition of sulfate reduction, as indicated by a decrease in hydrogen sulfide production relative to controls, resulted in a significant increase in H2 efflux, suggesting that sulfate-reducing bacteria (SRB) are important hydrogenotrophs. Low methane efflux under these same conditions indicated that methanogens are likely not important hydrogenotrophs. Analyses of genes and transcripts that encode for rRNA or dissimilatory sulfite reductase, using both PCR-dependent and PCR-independent metatranscriptomic sequencing methods, demonstrated that Desulfobacterales are the dominant, active SRB in the upper, H2-producing layer of the mat (0-2 mm). This hypothesis was further supported by the identification of transcripts encoding hydrogenases derived from Desulfobacterales capable of H2 oxidation. Analysis of molecular data provided no evidence for the activity of hydrogenotrophic methanogens. The combined biogeochemical and molecular data strongly indicate that SRB belonging to the Desulfobacterales are the quantitatively important hydrogenotrophs in the Elkhorn Slough mat. © 2014 John Wiley & Sons Ltd.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/85189
Appears in Collections:影响、适应和脆弱性

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作者单位: Departments of Chemical, Civil and Environmental Engineering, Stanford University, Stanford, CA, United States; Exobiology Branch, NASA Ames Research Center, Moffett Field, CA, United States; Earth Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, United States; Chemical Sciences Division, Lawrence Livermore National Laboratory, Livermore, CA, United States; Applied Chemistry and Biotechnology Section, Department of Industry, IP Australia, Canberra, Australia; Division of Microbial Ecology, Department of Microbiology and Ecosystem Science, University of Vienna, Vienna, Austria; Department of Bioscience, Center for Geomicrobiology, University of Aarhus, Aarhus, Denmark

Recommended Citation:
Burow L.C.,Woebken D.,Marshall I.P.G.,et al. Identification of Desulfobacterales as primary hydrogenotrophs in a complex microbial mat community[J]. Geobiology,2014-01-01,12(3)
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