DOI: 10.1016/j.marpolbul.2015.05.054
Scopus记录号: 2-s2.0-84956594653
论文题名: Impairment of mitochondrial energy metabolism of two marine fish by in vitro mercuric chloride exposure
作者: Mieiro C.L. ; Pardal M. ; Duarte A. ; Pereira E. ; Palmeira C.M.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2014
卷: 97, 期: 2018-01-02 起始页码: 488
结束页码: 493
语种: 英语
英文关键词: Enzymatic activities
; Marine fish
; Mercury
; Mitochondrial respiration
Scopus关键词: Biochemistry
; Chains
; Chlorine compounds
; Electron transport properties
; Mercury (metal)
; Mercury compounds
; Metabolism
; Mitochondria
; Phosphorylation
; Physiology
; Average concentration
; Electron transport chain
; Enzymatic activities
; Marine fish
; Mitochondrial function
; Mitochondrial respiration
; Oxidative phosphorylation
; Succinate dehydrogenase
; Fish
; adenosine triphosphatase
; mercuric chloride
; succinate dehydrogenase
; mercuric chloride
; water pollutant
; concentration (composition)
; enzyme activity
; fish
; health status
; inhibition
; mercury (element)
; metabolism
; mitochondrion
; pollution exposure
; respiration
; animal tissue
; anthropometric parameters
; Article
; chemical analysis
; concentration (parameters)
; controlled study
; disorders of mitochondrial functions
; electron transport
; energy metabolism
; health hazard
; health status
; juvenile animal
; liver metabolism
; mitochondrial respiration
; nonhuman
; oxidation
; oxygen consumption
; protein phosphorylation
; Solea senegalensis
; Sparus aurata
; toxicological parameters
; animal
; drug effects
; energy metabolism
; enzymology
; flatfish
; liver mitochondrion
; metabolism
; oxidation reduction reaction
; oxidative phosphorylation
; pharmacokinetics
; sea bream
; toxicity
; toxicity testing
; water pollutant
; Solea senegalensis
; Sparus aurata
; Animals
; Energy Metabolism
; Flatfishes
; Mercuric Chloride
; Mitochondria, Liver
; Oxidation-Reduction
; Oxidative Phosphorylation
; Oxygen Consumption
; Sea Bream
; Toxicity Tests
; Water Pollutants, Chemical
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: The goal of this work was to understand the extent of mercury toxic effects in liver metabolism under an episode of acute contamination. Hence, the effects of in vitro mercuric chloride in liver mitochondria were assessed in two commercial marine fish: Senegalese sole (Solea senegalensis) and gilthead seabream (Sparus aurata). Liver mitochondria were exposed to 0.2mgL-1 of mercury, the average concentration found in fish inhabiting contaminated areas. Mercuric chloride depressed mitochondrial respiration state 3 and the maximal oxygen consumption in the presence of FCCP indicating inhibitory effects on the oxidative phosphorylation and on the electron transport chain, respectively. The inhibition of F1Fo-ATPase and succinate-dehydrogenase activities also corroborated the ability of mercury to inhibit ADP phosphorylation and the electron transport chain. This study brings new understanding on the mercury levels able to impair fish mitochondrial function, reinforcing the need for further assessing bioenergetics as a proxy for fish health status. © 2015 Elsevier Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/85201
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: CESAM, Department of Chemistry, University of Aveiro, Campus de Santiago, Aveiro, Portugal; CFE, Department of Life Sciences, University of Coimbra, Apartado 3046, Coimbra, Portugal; CNC, Department of Life Sciences, University of Coimbra, Apartado 3046, Coimbra, Portugal
Recommended Citation:
Mieiro C.L.,Pardal M.,Duarte A.,et al. Impairment of mitochondrial energy metabolism of two marine fish by in vitro mercuric chloride exposure[J]. Marine Pollution Bulletin,2014-01-01,97(2018-01-02)