DOI: 10.1016/j.marpolbul.2014.04.002
Scopus记录号: 2-s2.0-84906238051
论文题名: Regulation of CYP11B1 and CYP11B2 steroidogenic genes by hypoxia-inducible miR-10b in H295R cells
作者: Nusrin S. ; Tong S.K.H. ; Chaturvedi G. ; Wu R.S.S. ; Giesy J.P. ; Kong R.Y.C.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2014
卷: 85, 期: 2 起始页码: 344
结束页码: 351
语种: 英语
英文关键词: Aldosterone
; Cortisol
; CYP11B1
; CYP11B2
; Hypoxia-inducible factor
; MiR-10b
Scopus关键词: Cells
; Cortisol
; Cytology
; Genes
; Aldosterone
; CYP11B1
; CYP11B2
; Hypoxia-inducible factors
; MiR-10b
; RNA
; aldosterone
; aldosterone synthase
; hydrocortisone
; hypoxia inducible factor 1alpha
; microRNA
; microRNA 10b
; aldosterone
; HIF1A protein, human
; hydrocortisone
; hypoxia inducible factor 1alpha
; messenger RNA
; MIRN10 microRNA, human
; steroid 11beta monooxygenase
; cell organelle
; experimental study
; gene expression
; hormone
; hypoxia
; RNA
; steroid
; 3' untranslated region
; adrenal cortex cell
; algorithm
; article
; cell line
; computer analysis
; computer model
; CYP11B1 gene
; cyp11b2 gene
; enzyme linked immunosorbent assay
; gene
; gene expression
; gene identification
; gene overexpression
; gene silencing
; gene targeting
; genetic regulation
; genetic transfection
; H295R cell line
; human
; human cell
; hypoxia
; luciferase assay
; protein expression
; real time polymerase chain reaction
; steroidogenesis
; validation process
; Western blotting
; cell hypoxia
; cell line
; computer simulation
; gene expression regulation
; genetics
; metabolism
; physiology
; Aldosterone
; Cell Hypoxia
; Cell Line
; Computer Simulation
; Cytochrome P-450 CYP11B2
; Gene Expression Regulation
; Gene Knockdown Techniques
; Humans
; Hydrocortisone
; Hypoxia-Inducible Factor 1, alpha Subunit
; MicroRNAs
; RNA, Messenger
; Steroid 11-beta-Hydroxylase
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: Although numerous studies have shown that hypoxia affects cortisol and aldosterone production in vivo, the underlying molecular mechanisms regulating the steroidogenic genes of these steroid hormones are still poorly known. MicroRNAs are post-transcriptional regulators that control diverse biological processes and this study describes the identification and validation of the hypoxia-inducible microRNA, miR-10b, as a negative regulator of the CYP11B1 and CYP11B2 steroidogenic genes in H295R human adrenocortical cells. Using the human TaqMan Low Density miRNA Arrays, we determined the miRNA expression patterns in H295R cells under normoxic and hypoxic conditions, and in cells overexpressing the human HIF-1α. Computer analysis using three in silico algorithms predicted that the hypoxia-inducible miR-10b molecule targets CYP11B1 and CYP11B2 mRNAs. Gene transfection studies of luciferase constructs containing the 3'-untranslated region of CYP11B1 or CYP11B2, combined with miRNA overexpression and knockdown experiments provide compelling evidence that CYP11B1 and CYP11B2 mRNAs are likely targets of miR-10b. © 2014 Elsevier Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/85295
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: Department of Biology and Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong; School of Biological Sciences, University of Hong Kong, Pokfulam, Hong Kong; Department of Veterinary Biomedical Sciences and Toxicology Centre, University of Saskatchewan, Canada; State Key Laboratory in Marine Pollution, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong
Recommended Citation:
Nusrin S.,Tong S.K.H.,Chaturvedi G.,et al. Regulation of CYP11B1 and CYP11B2 steroidogenic genes by hypoxia-inducible miR-10b in H295R cells[J]. Marine Pollution Bulletin,2014-01-01,85(2)