DOI: 10.1016/j.marpolbul.2014.05.053
Scopus记录号: 2-s2.0-84905740788
论文题名: The use of cholinesterase as potential biomarker: In vitro characterization in the polychaete Capitella teleta
作者: Gomes I.D.L. ; Lemos M.F.L. ; Soares A.M.V.M. ; Barata C. ; Faria M.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2014
卷: 85, 期: 1 起始页码: 179
结束页码: 185
语种: 英语
英文关键词: Acetylcholinesterase
; Capitela teleta
; Insecticide
; Marine
; Organophosphorous
; Sediment
Scopus关键词: Ecology
; Enzyme inhibition
; Fish
; Insecticides
; Marine engineering
; Sediments
; Acetylcholinesterase
; Biomonitoring studies
; Capitela teleta
; Cholinesterase activity
; Ecological relevance
; Ecotoxicological study
; Marine
; Organophosphorous
; Biomarkers
; 1,5 bis(4 allyldimethylammoniumphenyl)pentan 3 one dibromide
; acetylcholinesterase
; acetylthiocholine iodide
; biological marker
; butyrylthiocholine
; chlorpyrifos oxon
; choline derivative
; cholinesterase
; cholinesterase inhibitor
; eserine hemisulfate
; iodide
; iso OMPA
; propionylthiocholine iodide
; unclassified drug
; 1,5 bis(4 allyldimethylammoniumphenyl)pentan 3 one dibromide
; acetylthiocholine
; biological marker
; butyrylthiocholine
; chlorpyrifos
; cholinesterase
; iso OMPA
; O,O-diethyl O-3,5,6-trichloro-2-pyridyl phosphate
; physostigmine
; propionylthiocholine
; sulfate
; thiocholine
; annelid
; biomarker
; enzyme activity
; inhibition
; insecticide
; marine sediment
; polychaete
; adult
; animal tissue
; article
; biological monitoring
; Capitella teleta
; controlled study
; ecotoxicology
; enzyme activity
; enzyme analysis
; enzyme kinetics
; enzyme specificity
; enzyme substrate
; female
; IC 50
; in vitro study
; nonhuman
; polychaete
; sensitivity analysis
; worm
; analogs and derivatives
; animal
; drug effects
; environmental monitoring
; IC50
; metabolism
; Polychaeta
; procedures
; Capitella
; Polychaeta
; Acetylthiocholine
; Animals
; Benzenaminium, 4,4'-(3-oxo-1,5-pentanediyl)bis(N,N-dimethyl-N-2-propenyl-), Dibromide
; Biomarkers
; Butyrylthiocholine
; Chlorpyrifos
; Cholinesterase Inhibitors
; Cholinesterases
; Environmental Monitoring
; Inhibitory Concentration 50
; Physostigmine
; Polychaeta
; Sulfates
; Tetraisopropylpyrophosphamide
; Thiocholine
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: The ecological relevance of polychaetes coupled with their easy culture and maintenance in the laboratory, has led them to become increasingly used in marine ecotoxicological studies, raising the need to validate frequently applied monitoring tools at various biological levels. The present study was aimed to characterize the cholinesterases (ChE) activity in the polychaete Capitella teleta, using three substrates (acetylthiocholine iodide, propionylthiocholine iodide, and S-butyrylthiocholine iodide) and four known inhibitors (eserine hemisulfate, BW284c51, iso-OMPA and chlorpyrifos-oxon). Results showed that most of the measured cholinesterase activity was acetylcholinesterase (AChE). Inhibition of enzyme kinetic experiments denoted that sensitivity of C. teleta's ChE to the organophosphorous metabolite chlorpyrifos-oxon (IC50=60.72nM) was analogous to some fish species. This study highlights the relevance of ChE characterization before its use as a biomarker in ecotoxicology and biomonitoring studies. © 2014 Elsevier Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/85361
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: Environmental Chemistry Department, IDAEA-CSIC, Jordi Girona 18-26, 08034 Barcelona, Spain; Marine Resources Research Group, ESTM and GIRM, Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal; CESAM and Dept. Biology, University of Aveiro, 3810-193 Aveiro, Portugal
Recommended Citation:
Gomes I.D.L.,Lemos M.F.L.,Soares A.M.V.M.,et al. The use of cholinesterase as potential biomarker: In vitro characterization in the polychaete Capitella teleta[J]. Marine Pollution Bulletin,2014-01-01,85(1)