DOI: 10.1016/j.marpolbul.2014.06.018
Scopus记录号: 2-s2.0-84906101027
论文题名: Prevalence and genetic profiles of Escherichia coli from mangroves and mangrove associated foods off Goa, India
作者: Poharkar K.V. ; Kerkar S. ; Doijad S.P. ; Barbuddhe S.B.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2014
卷: 85, 期: 1 起始页码: 86
结束页码: 91
语种: 英语
英文关键词: Escherichia coli
; Mangrove
; PFGE
; Serotypes
Scopus关键词: Chemical analysis
; Electrophoresis
; Escherichia coli
; Genes
; Reservoirs (water)
; Sediments
; Mangrove
; Mangrove ecosystems
; PFGE
; Physicochemical parameters
; Potential reservoir
; Pulsed field gel electrophoresis
; Restriction digestion
; Serotypes
; Food microbiology
; digestion
; disease prevalence
; electrokinesis
; mangrove
; microbial activity
; pathogen
; physicochemical property
; point source pollution
; pollution monitoring
; toxic organism
; virulence
; article
; bacterial gene
; bacterial strain
; bacterial virulence
; bacterium isolation
; controlled study
; diarrhea
; ecosystem monitoring
; Escherichia coli
; food contamination
; food intake
; gene identification
; India
; mangrove
; microbial contamination
; molecular genetics
; nonhuman
; physical parameters
; prevalence
; pulsed field gel electrophoresis
; sediment
; serotyping
; stx1 gene
; stx2 gene
; water analysis
; Avicennia
; classification
; ecosystem
; Escherichia coli
; estuary
; food control
; gene expression profiling
; genetics
; geography
; human
; microbiology
; pH
; temperature
; Goa
; India
; Escherichia coli
; Rhizophoraceae
; Avicennia
; Diarrhea
; Ecosystem
; Electrophoresis, Gel, Pulsed-Field
; Escherichia coli
; Estuaries
; Food Microbiology
; Gene Expression Profiling
; Geography
; Geologic Sediments
; Humans
; Hydrogen-Ion Concentration
; India
; Serotyping
; Temperature
; Water Microbiology
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: A total of 120 samples comprising of water (45), sediment (45) and mangrove originated food (30) collected from mangrove ecosystems of Goa were screened for Escherichia coli employing ISO-16654 method. Seventy-one (59.16%) samples were positive for E. coli. The E. coli isolates were further characterized by serotyping, virulence gene profiling and pulsed field gel electrophoresis (PFGE). Water and sediment samples were analyzed for physico-chemical parameters. The serotypes reported were O1, O10, O13, O17, O36, O41, O50, O68, O105, O116, O141, O148, O159, O162 and rough types while, 23 strains could not be typed. The stx1 and stx2 genes were detected in 33(46.47%) and 16(22.53%) isolates, respectively. The XbaI restriction digestion patterns of the stx positive strains were diverse. Interestingly, few strains isolated from diarrheal patients and from water, sediment and food from mangrove sources were genetically similar. The study showed that the mangrove ecosystem could be a potential reservoir for pathogenic E. coli. © 2014 Elsevier Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/85377
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: ICAR Research Complex for Goa, Old Goa 403 402, India; Department of Biotechnology, Goa University, Taleigaon Plateau, Goa 403 206, India
Recommended Citation:
Poharkar K.V.,Kerkar S.,Doijad S.P.,et al. Prevalence and genetic profiles of Escherichia coli from mangroves and mangrove associated foods off Goa, India[J]. Marine Pollution Bulletin,2014-01-01,85(1)