DOI: 10.1016/j.marpolbul.2014.05.019
Scopus记录号: 2-s2.0-84903614810
论文题名: Marine phytoplankton motility sensor integrated into a microfluidic chip for high-throughput pollutant toxicity assessment
作者: Zheng G.-X. ; Li Y.-J. ; Qi L.-L. ; Liu X.-M. ; Wang H. ; Yu S.-P. ; Wang Y.-H.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2014
卷: 84, 期: 2018-01-02 起始页码: 147
结束页码: 154
语种: 英语
英文关键词: High-throughput
; Marine phytoplankton
; Microfluidic chip
; Motility
; Toxicity assessment
Scopus关键词: Fluidic devices
; Lead
; Marine biology
; Marine pollution
; Phenols
; Phytoplankton
; Pollution
; Sensors
; Testing
; High-throughput
; Marine phytoplankton
; Microfluidic chip
; Motility
; Toxicity assessment
; Toxicity
; copper
; lead
; mercury
; phenol
; assessment method
; concentration (composition)
; inhibition
; marine pollution
; motility
; phenol
; phytoplankton
; sensor
; toxicity
; article
; cell motility
; cell stimulation
; concentration response
; equipment design
; high throughput screening
; marine phytoplankton motility
; microfluidic analysis
; microfluidic chip
; nonhuman
; phytoplankton
; phytoplankton cell
; sea pollution
; toxicity testing
; velocity
; High-throughput
; Marine phytoplankton
; Microfluidic chip
; Motility
; Toxicity assessment
; Biological Assay
; Microfluidic Analytical Techniques
; Movement
; Oceans and Seas
; Phytoplankton
; Water Pollutants, Chemical
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: A microfluidic chip was designed to assess the toxicity of pollutants in a high-throughput way by using marine phytoplankton motility as a sensor signal. In this chip, multiple gradient generators (CGGs) with diffusible chambers enable large scale of dose-response bioassays to be performed in a simple way. Two mobile marine phytoplankton cells were confined on-chip and stimulated by 8 concentrations (generated by CGG) of Hg, Pb, Cu and phenol singly, as well as Cu and phenol jointly. CASA system was used to characterize motility by motile percentage (%MOT), curvilinear velocity (VCL), average path velocity (VAP) and straight line velocity (VSL). In all cases, dose-dependent inhibitions of motility were observed. In the present system, only 2h was needed to predict EC50. Thus, the developed microfluidic chip device was proved to be useful as a rapid/simple and high-throughput test method in marine pollution toxicity assessment. © 2014 Elsevier Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/85422
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: Chemical and Environmental Engineering Institute, Dalian University, Dalian, China; Medical School, Dalian University, Dalian, China; Microfluidic Research Institute, Dalian University, Dalian, China; Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China
Recommended Citation:
Zheng G.-X.,Li Y.-J.,Qi L.-L.,et al. Marine phytoplankton motility sensor integrated into a microfluidic chip for high-throughput pollutant toxicity assessment[J]. Marine Pollution Bulletin,2014-01-01,84(2018-01-02)