DOI: 10.1016/j.marpolbul.2014.03.047
Scopus记录号: 2-s2.0-84902285913
论文题名: Comparative molecular analysis of bacterial communities inhabiting pristine and polluted with polycyclic aromatic hydrocarbons Black Sea coastal sediments
作者: Todorova N.H. ; Mironova R.S. ; Karamfilov V.K.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2014
卷: 83, 期: 1 起始页码: 231
结束页码: 240
语种: 英语
英文关键词: 16S rRNA gene
; ARDRA
; Bacterial communities
; Oil pollution
; PAH degradative potential
; Sediment
Scopus关键词: Genes
; Polycyclic aromatic hydrocarbons
; RNA
; Sediments
; 16S rRNA gene
; 16s rrna gene libraries
; ARDRA
; Aromatic-ring-hydroxylating dioxygenase
; Bacterial community
; Bacterial community structure
; Molecular analysis
; Oil pollution
; Bacteria
; messenger RNA
; polycyclic aromatic hydrocarbon
; polycyclic aromatic hydrocarbon
; RNA 16S
; biodegradation
; coastal sediment
; community structure
; microbial community
; molecular analysis
; oil pollution
; PAH
; RNA
; sediment pollution
; Actinobacteria
; Alphaproteobacteria
; amplified ribosomal DNA restriction analysis
; article
; bacterium contamination
; biodegradability
; Black Sea
; comparative study
; controlled study
; Gammaproteobacteria
; gene library
; gene sequence
; microbial community
; microbial diversity
; nonhuman
; nucleotide sequence
; oil spill
; phylogenetic tree
; phylogeny
; prevalence
; sediment
; unindexed sequence
; Bacteria
; Black Sea
; chemistry
; classification
; DNA sequence
; genetics
; metabolism
; microbiology
; microflora
; oil spill
; restriction mapping
; sediment
; Black Sea
; Actinobacteria
; Bacteria (microorganisms)
; Gammaproteobacteria
; Bacteria
; Black Sea
; Gene Library
; Geologic Sediments
; Microbiota
; Petroleum Pollution
; Phylogeny
; Polycyclic Hydrocarbons, Aromatic
; Restriction Mapping
; RNA, Ribosomal, 16S
; Sequence Analysis, DNA
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: Molecular analysis was applied to characterize bacterial community structure in sediment samples collected from pristine site and oil-polluted Black Sea harbor. Amplified Ribosomal DNA Restriction Analysis (ARDRA) revealed a high similarity in the restriction patterns of both samples thus not demonstrating the effect of the pollutant on the structure of the bacterial communities. Constructed 16S rRNA gene libraries gave more detailed assessment of members. Results showed that α- and γ-Proteobacteria were dominant in the oil polluted site, whereas the pristine site was characterized by prevalence of Actinobacteria. The biodegradative potential of the adapted bacterial community in the oil-polluted sediments was demonstrated by the presence of the aromatic ring hydroxylating dioxygenase genes. © 2014 Elsevier Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/85490
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Y. Gagarin 2 str., 1113 Sofia, Bulgaria; Institute of Molecular Biology Roumen Tsanev, Bulgarian Academy of Sciences, G. Bonchev str., bl. 21, 1113 Sofia, Bulgaria
Recommended Citation:
Todorova N.H.,Mironova R.S.,Karamfilov V.K.. Comparative molecular analysis of bacterial communities inhabiting pristine and polluted with polycyclic aromatic hydrocarbons Black Sea coastal sediments[J]. Marine Pollution Bulletin,2014-01-01,83(1)