globalchange  > 过去全球变化的重建
DOI: 10.1016/j.marpolbul.2014.04.017
Scopus记录号: 2-s2.0-84902118255
论文题名:
Isolation of 12 microsatellite markers following a pyrosequencing procedure and cross-priming in two invasive cryptic species, Alexandrium catenella (group IV) and A. tamarense (group III) (Dinophyceae)
作者: Laporte M.; Shao Z.; Berrebi P.; Laabir M.; Abadie E.; Faivre N.; Rieuvilleneuve F.; Masseret E.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2014
卷: 83, 期:1
起始页码: 302
结束页码: 305
语种: 英语
英文关键词: Alexandrium ; Biogeography ; Dinophyceae ; Invasive species ; Microsatellite markers ; Phytoplankton
Scopus关键词: Climate change ; Ecology ; Phytoplankton ; Alexandrium ; Biogeography ; Dinophyceae ; Invasive species ; Microsatellite markers ; DNA sequences ; genomic DNA ; microsatellite DNA ; biogeography ; biomarker ; human activity ; invasive species ; isolation effect ; phytoplankton ; Alexandrium catenella ; Alexandrium tamarense ; article ; controlled study ; cross presentation ; dinoflagellate ; France ; gene amplification ; gene isolation ; genetic variability ; genotype ; haplotype ; lagoon ; Mediterranean Sea ; microsatellite marker ; nonhuman ; nucleotide sequence ; phytoplankton ; polymerase chain reaction ; population genetic structure ; pyrosequencing ; species distribution ; species diversity ; dinoflagellate ; DNA sequence ; genetics ; introduced species ; isolation and purification ; Etang de Thau ; France ; Herault ; Languedoc-Roussillon ; Mediterranean Sea ; Alexandrium ; Alexandrium catenella ; Alexandrium tamarense ; Dinophyceae ; Dinoflagellida ; Genetic Variation ; Introduced Species ; Mediterranean Sea ; Microsatellite Repeats ; Phytoplankton ; Sequence Analysis, DNA
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science ; Earth and Planetary Sciences: Oceanography ; Environmental Science: Pollution
英文摘要: •Alexandrium catenella (IV) and Alexandrium tamarense (III) are two cryptic invasive species.•Twelve new microsatellite markers were developed based of French strains.•Five markers showed amplifications on A. tamarense and ten on A. catenella.•Three allowed amplifications on both cryptic species.•These new markers will allow a better understanding of both species' biogeography. Alexandrium catenella (group IV) and Alexandrium tamarense (group III) (Dinophyceae) are two cryptic invasive phytoplankton species belonging to the A. tamarense species complex. Their worldwide spread is favored by the human activities, transportation and climate change. In order to describe their diversity in the Mediterranean Sea and understand their settlements and maintenances in this area, new microsatellite markers were developed based on Thau lagoon (France) samples of A. catenella and A. tamarense strains. In this study twelve new microsatellite markers are proposed. Five of these microsatellite markers show amplifications on A. tamarense and ten on A. catenella. Three of these 12 microsatellite markers allowed amplifications on both cryptic species. Finally, the haplotypic diversity ranged from 0.000 to 0.791 and 0.000 to 0.942 for A. catenella and A. tamarense respectively. © 2014.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/85491
Appears in Collections:过去全球变化的重建
全球变化的国际研究计划

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作者单位: Institut des Sciences de l'Evolution de Montpellier, UMR 5554 UM2-CNRS-IRD, Université Montpellier II, CC065, Place E. Bataillon, 34095 Montpellier, Cedex 5, France; Département de Biologie, IBIS (Institut de Biologie Intégrative et des Systèmes), Université Laval, Québec G1V 0A6, Canada; Ecologie des Systèmes Marins CÔtiers, UMR 5119 UM2-CNRS-IRD-Ifremer-UM1, Université Montpellier II, CC 093, 34095 Montpellier Cedex 5, France; Laboratoire Environnement Ressources Languedoc-Roussillon Ifremer, B.P. 171, 34203 Sète, France

Recommended Citation:
Laporte M.,Shao Z.,Berrebi P.,et al. Isolation of 12 microsatellite markers following a pyrosequencing procedure and cross-priming in two invasive cryptic species, Alexandrium catenella (group IV) and A. tamarense (group III) (Dinophyceae)[J]. Marine Pollution Bulletin,2014-01-01,83(1)
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