globalchange  > 过去全球变化的重建
DOI: 10.1016/j.marpolbul.2014.03.018
Scopus记录号: 2-s2.0-84899914065
论文题名:
Functional and molecular characterization of a lipopeptide surfactant from the marine sponge-associated eubacteria Bacillus licheniformis NIOT-AMKV06 of Andaman and Nicobar Islands, India
作者: Lawrance A.; Balakrishnan M.; Joseph T.C.; Palaiya Sukumaran D.; Nambali Valsalan V.; Gopal D.; Ramalingam K.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2014
卷: 82, 期:2018-01-02
起始页码: 76
结束页码: 85
语种: 英语
英文关键词: Andaman and Nicobar Islands ; Bacillus licheniformis ; Marine sponge ; Surfactant ; Surfactant biosynthesis genes
Scopus关键词: Bacteriology ; Biochemistry ; Bioremediation ; Biosynthesis ; Emulsification ; Enhanced recovery ; Escherichia coli ; Genes ; Hydrocarbon refining ; Hydrocarbons ; Andaman and Nicobar Islands ; Bacillus licheniformis ; Biosynthesis gene cluster ; Biosynthesis genes ; Enhanced oil recovery ; Lipopeptide surfactant ; Marine sponge ; Molecular characterization ; Surface active agents ; amylase ; casein ; catalase ; citric acid ; hydrocarbon ; lipopeptide ; oxidoreductase ; surfactant protein D ; triacylglycerol lipase ; bacterial DNA ; bacterial protein ; lipopeptide ; RNA 16S ; surfactant ; bioaccumulation ; concentration (composition) ; enhanced oil recovery ; environmental fate ; lichen ; marine pollution ; molecular analysis ; nitrogen ; oil pollution ; sponge ; surfactant ; amino acid substitution ; article ; Bacillus licheniformis ; Bacillus licheniformis NIOT AMKV06 ; bacterial gene ; bacterial growth ; bacterium isolate ; bioremediation ; carbon source ; emulsion ; enzyme activity ; enzyme synthesis ; Escherichia coli ; gene expression ; hemolysis ; India ; marine environment ; nonhuman ; nucleotide sequence ; pH ; protein synthesis ; salinity ; sfp gene ; sfpO gene ; srfA gene ; temperature ; thermostability ; amino acid sequence ; Bacillus ; chemistry ; genetics ; metabolism ; molecular genetics ; phylogeny ; Andamans ; Andamans and Nicobars ; India ; Nicobars ; Bacillus licheniformis ; Bacteria (microorganisms) ; Escherichia coli ; Amino Acid Sequence ; Bacillus ; Bacterial Proteins ; DNA, Bacterial ; India ; Lipopeptides ; Molecular Sequence Data ; Phylogeny ; RNA, Ribosomal, 16S ; Surface-Active Agents
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science ; Earth and Planetary Sciences: Oceanography ; Environmental Science: Pollution
英文摘要: The production of a lipopeptide surfactant from the sponge-associated eubacteria Bacillus licheniformis NIOT-AMKV06 from the Andaman and Nicobar Islands was investigated. The highest production was attained with glucose and yeast extracts as the carbon and nitrogen sources (1.789mgmL-1), respectively. The surfactant was highly stable over a pH range of 5.0-10 and a temperature range of 20-70°C with high NaCl concentrations. Excellent emulsification activity was exhibited by the purified surfactant with crude oil, kerosene, and diesel. A two-fold increase in surfactant production (3.0mgmL-1) was observed using the newly formulated medium in this study. The surfactant biosynthesis gene cluster (sfp, sfpO, and srfA) from B. licheniformis NIOT-AMKV06 was heterologously expressed in Escherichia coli, and the production was increased three-fold (11.78gL-1) over the original strain. The results confirm the potential of the surfactant for use in bioremediation of hydrocarbons in a marine environment and for enhanced oil recovery. To our knowledge, this is the first report on the ability of a hydrocarbon degrading B. licheniformis from marine sponges for the biosynthesis of a potent lipopeptide surfactant possessing characteristics of maximum stability, outstanding surfactant activity, and exceptional emulsifying capability. © 2014 Elsevier Ltd.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/85518
Appears in Collections:过去全球变化的重建
全球变化的国际研究计划

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作者单位: Andaman and Nicobar Centre for Ocean Science and Technology, Earth System Sciences Organization-National Institute of Ocean Technology (ESSO-NIOT), Port Blair-744 103, Andaman and Nicobar Islands, India; Marine Biotechnology Group, ESS0-NIOT, Ministry of Earth Sciences (Govt. of India), Chennai 600 100, India; Microbiology, Fermentation and Biotechnology Division, Central Institute of Fisheries Technology (CIFT), Indian Council of Agricultural Research (ICAR), Ministry of Agriculture (Govt. of India), Cochin 682 029, India

Recommended Citation:
Lawrance A.,Balakrishnan M.,Joseph T.C.,et al. Functional and molecular characterization of a lipopeptide surfactant from the marine sponge-associated eubacteria Bacillus licheniformis NIOT-AMKV06 of Andaman and Nicobar Islands, India[J]. Marine Pollution Bulletin,2014-01-01,82(2018-01-02)
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