DOI: 10.1016/j.marpolbul.2013.12.018
Scopus记录号: 2-s2.0-84893877892
论文题名: Enzymatic methods for the determination of pollution in seawater using salt resistant alkaline phosphatase from eggs of the sea urchin Strongylocentrotus intermedius
作者: Menzorova N.I. ; Seitkalieva A.V. ; Rasskazov V.A.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2014
卷: 79, 期: 2018-01-02 起始页码: 188
结束页码: 195
语种: 英语
英文关键词: Alkaline phosphatase (AP)
; Eggs of sea urchin
; Japan Sea
; Phosphatase inhibition assay
; Pollution monitoring
; Strongylocentrotus intermedius
Scopus关键词: Alkaline phosphatase (AP)
; Eggs of sea urchin
; Japan Sea
; Phosphatase inhibition assay
; Pollution monitoring
; Strongylocentrotus intermedius
; Alkaline Phosphatase
; Animals
; Environmental Monitoring
; Enzyme Assays
; Japan
; Metals
; Ovum
; Pesticides
; Seawater
; Sodium Chloride
; Strongylocentrotus
; Water Pollutants, Chemical
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: A new salt resistant alkaline phosphatase from eggs of the sea urchin Strongylocentrotus intermedius (StAP) has been shown to have a unique property to hydrolyze substrate in seawater without loss of enzymatic activity. The enzyme has pH optimum at 8.0-8.5. Model experiments showed various concentrations of copper, zinc, cadmium and lead added to seawater or a standard buffer mixture to inhibit completely the enzyme activity at the concentrations of 15-150. μg/l. StAP sensitivity to the presence in seawater of metals, pesticides, detergents and oil products appears to be considerably less. Samples of seawater taken from aquatic areas of the Troitsy Bay of the Peter the Great Bay, Japan Sea have been shown to inhibit the enzyme activity; the same was shown for the samples of fresh waters. The phosphatase inhibition assay developed proved to be highly sensitive, technically easy-to use allowing to test a great number of samples. © 2013 Elsevier Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/85614
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Science, Vladivostok, 159, Stoletya Vladivostoku Str., Vladivostok 690022, Russian Federation
Recommended Citation:
Menzorova N.I.,Seitkalieva A.V.,Rasskazov V.A.. Enzymatic methods for the determination of pollution in seawater using salt resistant alkaline phosphatase from eggs of the sea urchin Strongylocentrotus intermedius[J]. Marine Pollution Bulletin,2014-01-01,79(2018-01-02)