DOI: 10.1016/j.marpolbul.2014.09.018
Scopus记录号: 2-s2.0-84908615008
论文题名: Monitoring toxic Ostreopsis cf. ovata in recreational waters using a qPCR based assay
作者: Casabianca S. ; Perini F. ; Casabianca A. ; Battocchi C. ; Giussani V. ; Chiantore M. ; Penna A.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2014
卷: 88, 期: 2018-01-02 起始页码: 102
结束页码: 109
语种: 英语
英文关键词: HABs
; Monitoring
; Ostreopsis
; QPCR
; Recreational waters
; Toxic algae
Scopus关键词: Algae
; Aquaculture
; Assays
; Genes
; Health risks
; Monitoring
; Environmental sample
; Environmental standards
; HABs
; Ostreopsis
; Preventive measures
; QPCR
; Recreational water
; Species-specific identification
; Polymerase chain reaction
; large subunit ribosomal DNA
; molecular marker
; ribosome DNA
; sea water
; unclassified drug
; cytochrome c oxidase
; marine toxin
; protozoal protein
; ribosome DNA
; sea water
; algal bloom
; bioassay
; biomass
; biomonitoring
; dinoflagellate
; gene
; mitochondrial DNA
; pollution monitoring
; polymerase chain reaction
; toxic organism
; Article
; coastal waters
; cob gene
; COI gene
; controlled study
; dinoflagellate
; DNA sequence
; environmental monitoring
; gene
; gene dosage
; geographic distribution
; limit of quantitation
; Mediterranean Sea
; nonhuman
; Ostreopsis cf. ovata
; polymerase chain reaction
; population abundance
; quantitative analysis
; sensitivity analysis
; species difference
; species identification
; summer
; water sampling
; algal bloom
; dinoflagellate
; environmental monitoring
; genetics
; human
; limit of detection
; parasitology
; plasmid
; procedures
; real time polymerase chain reaction
; recreation
; Southern Europe
; water quality
; Mediterranean Sea
; algae
; Ostreopsis
; Ostreopsis ovata
; Dinoflagellida
; DNA, Ribosomal
; Electron Transport Complex IV
; Environmental Monitoring
; Gene Dosage
; Harmful Algal Bloom
; Humans
; Limit of Detection
; Marine Toxins
; Mediterranean Region
; Plasmids
; Protozoan Proteins
; Real-Time Polymerase Chain Reaction
; Recreation
; Seawater
; Water Quality
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: Ostreopsis sp. is a toxic marine benthic dinoflagellate that causes high biomass blooms, posing a threat to human health, marine biota and aquaculture activities, and negatively impacting coastal seawater quality. Species-specific identification and enumeration is fundamental because it can allow the implementation of all the necessary preventive measures to properly manage Ostreopsis spp. bloom events in recreational waters and aquaculture farms. The aim of this study was to apply a rapid and sensitive qPCR method to quantify Ostreopsis cf. ovata abundance in environmental samples collected from Mediterranean coastal sites and to develop site-specific environmental standard curves. Similar PCR efficiencies of plasmid and environmental standard curves allowed us to estimate the LSU rDNA copy number per cell. Moreover, we assessed the effectiveness of mitochondrial COI and cob genes as alternative molecular markers to ribosomal genes in qPCR assays for Ostreopsis spp. quantification. © 2014 Elsevier Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/85769
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: Section of Environmental Biology, Dep. of Biomolecular Sciences, University of Urbino, Viale Trieste 296, Pesaro, Italy; Department of Earth, Environment and Life Sciences, University of Genoa, Corso Europa 26, 16132, Italy; ISMAR CNR, Largo Fiera della Pesca, Ancona, Italy
Recommended Citation:
Casabianca S.,Perini F.,Casabianca A.,et al. Monitoring toxic Ostreopsis cf. ovata in recreational waters using a qPCR based assay[J]. Marine Pollution Bulletin,2014-01-01,88(2018-01-02)