DOI: 10.1016/j.marpolbul.2014.08.025
Scopus记录号: 2-s2.0-84908617248
论文题名: Molecular biomarkers in grey seals (Halichoerus grypus) to evaluate pollutant exposure, health and immune status
作者: Lehnert K. ; Müller S. ; Weirup L. ; Ronnenberg K. ; Pawliczka I. ; Rosenberger T. ; Siebert U.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2014
卷: 88, 期: 2018-01-02 起始页码: 311
结束页码: 318
语种: 英语
英文关键词: Halichoerus grypus
; Immune function
; MRNA biomarker
; Multiple stresses
; Pollution effects
; Xenobiotic metabolism
Scopus关键词: Health
; Impurities
; Metabolism
; Physiology
; Pollution
; Polymerase chain reaction
; Transcription
; Halichoerus grypus
; Immune function
; Multiple stress
; Pollution effects
; Xenobiotic metabolism
; Biomarkers
; aromatic hydrocarbon receptor
; biological marker
; heat shock protein 70
; interleukin 2
; messenger RNA
; peroxisome proliferator activated receptor alpha
; transcription factor ARNTL
; aromatic hydrocarbon receptor
; biological marker
; dangerous goods
; genetic marker
; heat shock protein 70
; hypoxia inducible factor 1beta
; interleukin 2
; peroxisome proliferator activated receptor alpha
; water pollutant
; xenobiotic agent
; bioaccumulation
; biomarker
; health status
; immune system
; pinniped
; pollution effect
; pollution exposure
; xenobiotics
; adult
; age
; animal health
; Article
; aryl hydrocarbon receptor gene
; body weight
; controlled study
; environmental exposure
; environmental stress
; gene expression
; Halichoerus grypus
; health status
; heat shock protein 70 gene
; immune status
; immune system
; interleukin 2 gene
; nonhuman
; nucleotide sequence
; peroxisome proliferator activated receptor alpha gene
; reverse transcription polymerase chain reaction
; seal
; species difference
; transcription factor ARNTL gene
; xenobiotic metabolism
; analysis
; animal
; blood
; dangerous goods
; environmental monitoring
; female
; food chain
; genetic marker
; genetics
; human
; immunology
; metabolism
; Phocidae
; physiology
; procedures
; water pollutant
; Halichoerus grypus
; Animals
; Aryl Hydrocarbon Receptor Nuclear Translocator
; Biological Markers
; Environmental Monitoring
; Female
; Food Chain
; Genetic Markers
; Hazardous Substances
; HSP70 Heat-Shock Proteins
; Humans
; Interleukin-2
; PPAR alpha
; Receptors, Aryl Hydrocarbon
; Seals, Earless
; Water Pollutants, Chemical
; Xenobiotics
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: Grey seals as top-predators bioaccumulate contaminants and can be considered as sentinels of eco-system health. Pups are weaned after a short nursing period, characterised by an enormous lipid transfer and exposure to contaminants. This study established molecular biomarkers of the xenobiotic metabolism and immune system to help assess health and immune status. mRNA transcription of AHR, ARNT, PPARα and cytokine IL-2 and heat-shock-protein HSP70 was measured in blood of grey seal pups and adults in rehabilitation and permanent care using RT-qPCR and compared to rehabilitating harbour seal pups and haematology values. In pups highest levels at admission in xenobiotic biomarker, HSP70 and cytokine transcription may show contaminant exposure via lactation, stress during abandonment and dehydration. The significant decrease may be linked to diet, health improvement and adaptation. Adults showed higher levels and more variation in biomarker transcription and clear species-specific differences between harbour and grey seal pups were found. © 2014 Elsevier Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/85847
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: Institute for Terrestrial and Aquatic Wildlife Research, University of Veterinary Medicine Hannover, Büsum, Germany; Institute for Coastal Research, Helmholtz-Zentrum Geesthacht Centre for Materials and Coastal Research, Geesthacht, Germany; Hel Marine Station, University of Gdansk, Morska 2, Hel, Poland; Seal Centre Friedrichskoog, An der Seeschleuse 4, Friedrichskoog, Germany
Recommended Citation:
Lehnert K.,Müller S.,Weirup L.,et al. Molecular biomarkers in grey seals (Halichoerus grypus) to evaluate pollutant exposure, health and immune status[J]. Marine Pollution Bulletin,2014-01-01,88(2018-01-02)