DOI: 10.1016/j.marpolbul.2014.07.008
Scopus记录号: 2-s2.0-84922620498
论文题名: Antibiotic resistance of Vibrio harveyi isolated from seawater in Korea
作者: Kang C.-H. ; Kim Y. ; Oh S.J. ; Mok J.-S. ; Cho M.-H. ; So J.-S.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2014
卷: 86, 期: 2018-01-02 起始页码: 261
结束页码: 265
语种: 英语
英文关键词: Isolation
; Multiple antibiotic resistance (MAR)
; Seawater
; Vibrio harveyi
Scopus关键词: Antibiotics
; RNA
; Shellfish
; Antibiotic resistance
; Biochemical assay
; Isolation
; Multiple antibiotic resistances
; Seawater samples
; Soft tissue infections
; Species specifics
; Vibrio harveyi
; Seawater
; agar
; ampicillin
; bile salt
; cefalotin
; cefepime
; cefotaxime
; cefotetan
; chloramphenicol
; ciprofloxacin
; citric acid
; cotrimoxazole
; erythromycin
; gentamicin
; hemolysin
; kanamycin
; nalidixic acid
; rifampicin
; RNA 16S
; sea water
; streptomycin
; sucrose
; tetracycline
; thiosulfate
; vancomycin
; antiinfective agent
; primer DNA
; antibiotic resistance
; aquaculture
; Article
; assay
; bacterial strain
; bacterium colony
; bacterium isolate
; bacterium isolation
; controlled study
; diagnostic kit
; incubation time
; nonhuman
; polymerase chain reaction
; sea
; shellfish
; South Korea
; species difference
; Vibrio harveyi
; water sampling
; antibiotic resistance
; drug effects
; genetics
; microbiology
; Vibrio
; Negibacteria
; Vibrio harveyi
; Anti-Bacterial Agents
; DNA Primers
; Drug Resistance, Microbial
; Hemolysin Proteins
; Polymerase Chain Reaction
; Republic of Korea
; RNA, Ribosomal, 16S
; Seawater
; Species Specificity
; Vibrio
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: Vibrio harveyi is an opportunistic human pathogen that may cause gastroenteritis, severe necrotizing soft-tissue infections, and primary septicemia, with a potentially high rate of lethality. In this study, we isolated and characterized V. harveyi from seawater collected from the West Sea in Korea, including sites located near shellfish farms. For the initial isolation of putative V. harveyi, isolates were incubated on thiosulfate citrate bile salt sucrose agar plates for 24. h, followed by selection of greenish colonies. Gram-negative and oxidase-positive colonies were subsequently confirmed by biochemical assays and the API 20E kit test system. Species-specific 16S rRNA and hemolysin genes were used to design V. harveyi-specific PCR primers. From 840 seawater samples, a total of 2 strains of V. harveyi were isolated from shellfish farm seawater. The two isolates were subjected to profiling against 16 antibiotics and found to be resistant to cephalothin, vancomycin, ampicillin, cefepime, cefotetan, and streptomycin. © 2014 Elsevier Ltd.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/85867
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: Department of Biological Engineering, Inha University, Incheon, South Korea; Food Safety Research Division, National Fisheries Research and Development Institute, Busan, South Korea; Department of Biological Sciences, Konkuk University, Seoul, South Korea
Recommended Citation:
Kang C.-H.,Kim Y.,Oh S.J.,et al. Antibiotic resistance of Vibrio harveyi isolated from seawater in Korea[J]. Marine Pollution Bulletin,2014-01-01,86(2018-01-02)