DOI: 10.1016/j.marpolbul.2015.04.038
Scopus记录号: 2-s2.0-84930752630
论文题名: Proteomic analysis of the European flounder Platichthys flesus response to experimental PAH-PCB contamination
作者: Galland C. ; Dupuy C. ; Loizeau V. ; Danion M. ; Auffret M. ; Quiniou L. ; Laroche J. ; Pichereau V.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2015
卷: 95, 期: 2 起始页码: 646
结束页码: 657
语种: 英语
英文关键词: Betaine and methionine metabolism
; Biomarker
; Persistent organic pollutants
; Platichthys flesus
; Pollution
; Proteomics
Scopus关键词: Amino acids
; Betaines
; Biochemistry
; Biomarkers
; Mass spectrometry
; Metabolism
; Molecular biology
; Physiology
; Pollution
; Polychlorinated biphenyls
; Polycyclic aromatic hydrocarbons
; Proteins
; Water quality
; Accumulation factors
; Glutathione metabolism
; Molecular mechanism
; Persistent organic pollutant (POP)
; Platichthys flesus
; Proteomic analysis
; Proteomic approaches
; Proteomics
; Organic pollutants
; betaine
; methionine
; polychlorinated biphenyl
; polycyclic aromatic hydrocarbon
; polychlorinated biphenyl
; polycyclic aromatic hydrocarbon
; proteome
; water pollutant
; xenobiotic agent
; bioaccumulation
; biomarker
; estuarine pollution
; flatfish
; metabolism
; PAH
; PCB
; persistent organic pollutant
; proteomics
; water quality
; animal tissue
; Article
; bioaccumulation
; contamination
; controlled study
; demethylation
; estuary
; glutathione metabolism
; indicator organism
; matrix assisted laser desorption ionization time of flight mass spectrometry
; nonhuman
; oxidative stress
; Platichthys flesus
; protein determination
; proteomics
; analysis
; animal
; environmental monitoring
; flounder
; liver
; metabolism
; physiology
; proteomics
; toxicity
; water pollutant
; France
; Seine Estuary
; Platichthys flesus
; Animals
; Environmental Monitoring
; Estuaries
; Flounder
; Liver
; Polychlorinated Biphenyls
; Polycyclic Hydrocarbons, Aromatic
; Proteome
; Proteomics
; Water Pollutants, Chemical
; Xenobiotics
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: Platichthys flesus is often used as a sentinel species to monitor the estuarine water quality. In this study, we carried out an experimental contamination of fish using a PAHs/PCBs mixture, which was designed to mimic the concentrations found in the Seine estuary (C1) and 10 times these concentrations (C2). We used a proteomic approach to understand the molecular mechanisms implied in the response of P. flesus to these xenobiotics. We showed that 54 proteins were differentially accumulated in one or several conditions, which 34 displayed accumulation factors higher than two. 18 of these proteins were identified by MALDI TOF-TOF mass spectrometry. The results indicated the deregulation of oxidative stress- and glutathione metabolism-(GST, GPx) proteins as well as of several proteins belonging to the betaine demethylation pathway and the methionine cycle (BHMT, SHMT, SAHH), suggesting a role for these different pathways in the P. flesus response to chemical contamination. © 2015 Elsevier Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/86031
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: Université de Brest, Laboratoire des Sciences de l'Environnement Marin, LEMAR, UMR 6539 UBO/CNRS/IRD/Ifremer, Institut Universitaire Européen de la Mer (IUEM), Plouzané, France; Unité de Biogéochimie et Ecotoxicologie, IFREMER, Centre de Brest, BP 70, Plouzané, France; ANSES, Agence nationale de securite sanitaire de l'alimentation et de l'environnement et du travail, site de Ploufragan-Plouzané-Technopole Brest Iroise, Plouzané, France
Recommended Citation:
Galland C.,Dupuy C.,Loizeau V.,et al. Proteomic analysis of the European flounder Platichthys flesus response to experimental PAH-PCB contamination[J]. Marine Pollution Bulletin,2015-01-01,95(2)