DOI: 10.1016/j.marpolbul.2015.03.043
Scopus记录号: 2-s2.0-84930759250
论文题名: In vitro and in vivo studies of the endocrine disrupting potency of cadmium in roach (Rutilus rutilus) liver
作者: Gerbron M. ; Geraudie P. ; Xuereb B. ; Marie S. ; Minier C.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2015
卷: 95, 期: 2 起始页码: 582
结束页码: 589
语种: 英语
英文关键词: Cadmium
; Endocrine disruption
; Liver explants
; Roach
; Steroid receptors
; Vitellogenin
Scopus关键词: Biosynthesis
; Cadmium
; Endocrine disruption
; Liver explants
; Roach
; Steroid receptors
; Vitellogenin
; Endocrine disrupters
; androgen receptor
; cadmium chloride
; endocrine disruptor
; estrogen receptor alpha
; estrogen receptor beta
; estrogen receptor beta 1
; messenger RNA
; unclassified drug
; vitellogenin
; androgen receptor
; cadmium
; endocrine disruptor
; estradiol
; estrogen receptor
; estrogen receptor alpha
; messenger RNA
; water pollutant
; bioaccumulation
; cadmium
; cyprinid
; endocrine disruptor
; protein
; RNA
; steroid
; animal experiment
; animal tissue
; Article
; cell viability
; controlled study
; ex vivo study
; fish
; gene expression
; in vitro study
; in vivo study
; liver
; male
; nonhuman
; reproduction
; Rutilus rutilus
; animal
; Cyprinidae
; metabolism
; physiology
; toxicity
; toxicity testing
; water pollutant
; Rutilus rutilus
; Vertebrata
; Animals
; Cadmium
; Cyprinidae
; Endocrine Disruptors
; Estradiol
; Estrogen Receptor alpha
; In Vitro Techniques
; Liver
; Receptors, Androgen
; Receptors, Estrogen
; RNA, Messenger
; Toxicity Tests
; Vitellogenins
; Water Pollutants, Chemical
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: Cadmium has been reported to exert estrogenic, antiestrogenic or both effects in vertebrate species. To elucidate the endocrine disrupting action of CdCl2, ex vivo and in vivo experiments were performed in roach (Rutilus rutilus). Roach liver explants were exposed to a range of CdCl2 concentrations alone (0.1-50μM) or with an effective concentration (100nM) of 17β-estradiol (E2). In addition, juvenile roach were intraperitoneally injected with CdCl2 (0.1-2.5mg/kg) with or without 1mg E2/kg. Subsequent analysis evaluated the effect of CdCl2 on vitellogenin (VTG) synthesis both at the mRNA and protein level, on estrogen receptors (erα and erβ1) and on androgen receptor (ar) mRNA expression. Ex vivo and in vivo experiments indicated that CdCl2 is strongly anti-estrogenic as, when co-exposed to E2, CdCl2 significantly inhibited VTG production as well as vtg and erα mRNA expressions. Moreover, CdCl2 compromised the E2-mediated induction of the ar mRNA expression in vivo. © 2015 Elsevier Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/86033
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: Laboratory of Ecotoxicology, ULH Normandie Université, SFR SACLE 4116, BP 540, Le Havre, France; Akvaplan Niva as, Fram Centre, Tromsø, Norway
Recommended Citation:
Gerbron M.,Geraudie P.,Xuereb B.,et al. In vitro and in vivo studies of the endocrine disrupting potency of cadmium in roach (Rutilus rutilus) liver[J]. Marine Pollution Bulletin,2015-01-01,95(2)