globalchange  > 过去全球变化的重建
DOI: 10.1016/j.marpolbul.2015.04.027
Scopus记录号: 2-s2.0-84933679193
论文题名:
Effects of low seawater pH on the marine polychaete Platynereis dumerilii
作者: Wäge J.; Hardege J.D.; Larsson T.A.; Simakov O.; Chapman E.C.; Arendt D.; Rotchell J.M.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2015
卷: 95, 期:1
起始页码: 166
结束页码: 172
语种: 英语
英文关键词: Acid-base regulation ; Gene expression ; Low pH ; P. dumerilii ; Polychaeta
Scopus关键词: Cams ; Carbonic anhydrase ; Gene expression ; Genes ; Mobile security ; Seawater ; Seawater effects ; Acid base ; Acid-base balance ; Acidified seawaters ; Cellular homeostasis ; Environmental conditions ; Molecular levels ; P. dumerilii ; Polychaeta ; Gene expression regulation ; bicarbonate ; calmodulin ; carbon dioxide ; carbonate dehydratase ; messenger RNA ; sea water ; sodium proton exchange protein ; calmodulin ; carbonate dehydratase ; RNA 18S ; sodium proton exchange protein ; acidification ; detection method ; environmental factor ; gene expression ; homeostasis ; pH ; polychaete ; RNA ; seawater ; acidification ; Article ; CA gene ; CaM gene ; controlled study ; down regulation ; environmental exposure ; gene ; gene expression ; NHE gene ; nonhuman ; pH measurement ; Platynereis dumerilii ; Polychaeta ; upregulation ; animal ; chemistry ; gene expression regulation ; genetics ; pH ; physiology ; Polychaeta ; Platynereis dumerilii ; Polychaeta ; Animals ; Calmodulin ; Carbonic Anhydrases ; Gene Expression Regulation ; Hydrogen-Ion Concentration ; Polychaeta ; RNA, Ribosomal, 18S ; Seawater ; Sodium-Hydrogen Antiporter
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science ; Earth and Planetary Sciences: Oceanography ; Environmental Science: Pollution
英文摘要: An important priority for any organism is to maintain internal cellular homeostasis including acid-base balance. Yet, the molecular level impacts of changing environmental conditions, such as low pH, remain uncharacterised. Herein, we isolate partial Na+/H+ exchangers (NHE), carbonic anhydrase (CA), and calmodulin (CaM) genes from a polychaete, Platynereis dumerilii and investigate their relative expression in acidified seawater conditions. mRNA expression of NHE was significantly down-regulated after 1h and up-regulated after 7days under low pH treatment (pH 7.8), indicating changes in acid-base transport. Furthermore, the localisation of NHE expression was also altered. A trend of down regulation in CA after 1h was also observed, suggesting a shift in the CO2 and HCO3 - balance. No change in CaM expression was detected after 7days exposure to acidified seawater. This study provides insight into the molecular level changes taking place following exposure to acidified seawater in a non-calcifying, ubiquitous, organism. © 2015 Elsevier Ltd.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/86049
Appears in Collections:过去全球变化的重建
全球变化的国际研究计划

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作者单位: School of Biological, Biomedical and Environmental Sciences, University of Hull, Cottingham Road, Hull, United Kingdom; European Molecular Biology Laboratory, Heidelberg, Meyerhofstr. 1, Heidelberg, Germany

Recommended Citation:
Wäge J.,Hardege J.D.,Larsson T.A.,et al. Effects of low seawater pH on the marine polychaete Platynereis dumerilii[J]. Marine Pollution Bulletin,2015-01-01,95(1)
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