DOI: 10.1016/j.marpolbul.2015.06.029
Scopus记录号: 2-s2.0-84956798181
论文题名: Validation of algal viability treated with total residual oxidant and organic matter by flow cytometry
作者: Lee J. ; Choi E.J. ; Rhie K.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2015
卷: 97, 期: 2018-01-02 起始页码: 95
结束页码: 104
语种: 英语
英文关键词: Algal viability
; ATP contents
; Ballast water management
; Flow cytometry
; Organic matter
; Total residual oxidant
Scopus关键词: Biogeochemistry
; Biological materials
; Cells
; Chlorine compounds
; Cytology
; Flow cytometry
; Organic compounds
; Starch
; Water
; Algal viability
; ATP content
; Ballast water management
; Fluorescein diacetate
; International maritime organizations
; Phaeodactylum tricornutum
; Selenastrum capricornutum
; Total residual oxidants
; Oxidants
; adenosine triphosphate
; chlorine
; fluorescein diacetate
; hypochlorite sodium
; organic matter
; sodium thiosulfate
; starch
; 3',6'-diacetylfluorescein
; adenosine triphosphate
; fluorescein derivative
; fresh water
; oxidizing agent
; sea water
; starch
; ballast water
; diatom
; flow cytometry
; green alga
; growth response
; marine pollution
; microalga
; organic matter
; water management
; Acutodesmus obliquus
; algal cell culture
; aquatic species
; Article
; Ballast Water Management Convention
; cell count
; cell growth
; cell viability
; controlled study
; flow cytometry
; Isochrysis galbana
; nonhuman
; Phaeodactylum tricornutum
; Selenastrum capricornutum
; staining
; water management
; chemistry
; cytology
; drug effects
; flow cytometry
; green alga
; Haptophyta
; metabolism
; procedures
; Scenedesmus
; ship
; species difference
; toxicity testing
; validation study
; algae
; Isochrysis galbana
; Phaeodactylum tricornutum
; Scenedesmus obliquus
; Selenastrum capricornutum
; Adenosine Triphosphate
; Chlorophyta
; Flow Cytometry
; Fluoresceins
; Fresh Water
; Haptophyta
; Oxidants
; Scenedesmus
; Seawater
; Ships
; Species Specificity
; Starch
; Toxicity Tests
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: Algal cell growth after starch and oxidant treatments in seawater species (Isochrysis galbana and Phaeodactylum tricornutum) and freshwater species (Selenastrum capricornutum and Scenedesmus obliquus) were evaluated by flow cytometry with fluorescein diacetate (FDA) staining to determine algal viability. Growth of algal cell was found to be significantly different among groups treated with NaOCl, starch and/or sodium thiosulfate, which are active substance (Total Residual Oxidant; TRO as Cl2), organic compound to meet efficacy testing standard and neutralizer of TRO by Ballast Water Management Convention of International Maritime Organization, respectively. The viability of algal cell treated with TRO in starch-add culture of 5days after treatment and neutralization was decreased significantly. ATP contents of the treated algal cells corresponded to the FL1 fluorescent signal of flow cytometry with FDA staining. I. galbana was the most sensitive to TRO-neutralized cultures during viability analysis. © 2015 Elsevier Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/86136
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: Department of Biology, Kyung Hee University, Seoul, South Korea
Recommended Citation:
Lee J.,Choi E.J.,Rhie K.. Validation of algal viability treated with total residual oxidant and organic matter by flow cytometry[J]. Marine Pollution Bulletin,2015-01-01,97(2018-01-02)