DOI: 10.1016/j.marpolbul.2014.12.010
Scopus记录号: 2-s2.0-84964284490
论文题名: The use of flow cytometric applications to measure the effects of PAHs on growth, membrane integrity, and relative lipid content of the benthic diatom, Nitzschia brevirostris
作者: Croxton A.N. ; Wikfors G.H. ; Schulterbrandt-Gragg R.D. ; III
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2015
卷: 91, 期: 1 起始页码: 160
结束页码: 165
语种: 英语
英文关键词: Algal cellular physiology
; Fluorescent probes
; Membrane permeability
; Microphytobenthos
; Oil pollution
; Rapid method
Scopus关键词: Aromatic hydrocarbons
; Cells
; Cytology
; Fluorescence
; Naphthalene
; Oils and fats
; Organic pollutants
; Physiology
; Pollution
; Pyrene
; Cellular physiology
; Fluorescent probes
; Membrane permeability
; Microphytobenthos
; Oil pollution
; Rapid method
; Polycyclic aromatic hydrocarbons
; benzo[a]pyrene
; fluorescent dye
; lipid
; naphthalene
; polycyclic aromatic hydrocarbon
; pyrene
; drug analysis
; drug toxicity
; lipid
; naphthalene derivative
; pollutant
; polycyclic aromatic hydrocarbon
; pyrene derivative
; diatom
; food web
; growth response
; lipid
; membrane
; oil pollution
; organic pollutant
; PAH
; physiological response
; phytobenthos
; Article
; cell count
; cell density
; cell growth
; cell membrane
; cell viability
; concentration (parameters)
; controlled study
; diatom
; exposure
; flow cytometry
; lipid storage
; Nitzschia brevirostris
; nonhuman
; pollutant
; population density
; analysis
; cell membrane
; diatom
; drug effects
; evaluation study
; flow cytometry
; fluorescence
; growth, development and aging
; metabolism
; procedures
; toxicity
; algae
; Bacillariophyta
; Nitzschia brevirostris
; analysis
; analysis
; analysis
; analysis
; analysis
; analysis
; Benzo(a)pyrene
; Cell Membrane
; Diatoms
; drug effects
; drug effects
; Environmental Pollutants
; Flow Cytometry
; Fluorescence
; growth and development
; Lipids
; metabolism
; methods
; Naphthalenes
; Polycyclic Hydrocarbons, Aromatic
; Population Density
; Pyrenes
; toxicity
; toxicity
; toxicity
; toxicity
; toxicity
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: This laboratory study measured the direct effects of three polycyclic aromatic hydrocarbon (PAH) compounds (naphthalene, pyrene, and benzo(a)pyrene) upon cell growth, membrane integrity, and BODIPY-stained lipid fluorescence intensity of the benthic diatom Nitzschia brevirostris using flow cytometry as an analysis tool. Previous field and laboratory studies have reported reductions in algal populations following PAH exposure, but specific, functional responses of the microalgae to these pollutants could not be revealed by cell numbers alone. Using flow-cytometric measurements, we confirmed that maximal cell densities in PAH-exposed diatom cultures were significantly lower compared to controls; however, we also discovered increases in lipids and cells with compromised membranes in PAH-exposed cultures. These results highlight new tools for measuring the direct effects of organic pollutants upon the physiology of taxa comprising microphytobenthic communities important in estuarine food webs. © 2014. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/86251
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: Northeast Fisheries Science Center, NMFS, NOAA, 212 Rogers Avenue, Milford, CT, United States; School of the Environment, Florida A andM University, 1520 S. Bronough St., Tallahassee, FL, United States
Recommended Citation:
Croxton A.N.,Wikfors G.H.,Schulterbrandt-Gragg R.D.,et al. The use of flow cytometric applications to measure the effects of PAHs on growth, membrane integrity, and relative lipid content of the benthic diatom, Nitzschia brevirostris[J]. Marine Pollution Bulletin,2015-01-01,91(1)