DOI: 10.1016/j.marpolbul.2015.01.008
Scopus记录号: 2-s2.0-84925321348
论文题名: Metabarcoding approach for the ballast water surveillance - An advantageous solution or an awkward challenge?
作者: Zaiko A. ; Martinez J.L. ; Schmidt-Petersen J. ; Ribicic D. ; Samuiloviene A. ; Garcia-Vazquez E.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2015
卷: 92, 期: 2018-01-02 起始页码: 25
结束页码: 34
语种: 英语
英文关键词: Ballast water management convention
; COI
; En-route observation
; High-throughput sequencing
; Invasive species
; RuBisCO
Scopus关键词: Biodiversity
; Genes
; Monitoring
; Ballast water management
; COI
; En-route
; High-throughput sequencing
; Invasive species
; RuBisCo
; Water
; ballast water
; unclassified drug
; water
; cytochrome c oxidase
; ribulosebisphosphate carboxylase
; water
; ballast water
; biodiversity
; genetic marker
; invasive species
; molecular analysis
; performance assessment
; pollution monitoring
; water management
; Article
; biological monitoring
; controlled study
; cost effectiveness analysis
; cytochrome oxidase I gene
; gene
; intermethod comparison
; introduced species
; metabarcoding
; nonhuman
; species diversity
; species identification
; water analysis
; water sampling
; animal
; biodiversity
; DNA barcoding
; genetics
; microbiology
; procedures
; ship
; temperature
; Animals
; Biodiversity
; DNA Barcoding, Taxonomic
; Electron Transport Complex IV
; Introduced Species
; Ribulose-Bisphosphate Carboxylase
; Ships
; Temperature
; Water
; Water Microbiology
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: Transfer of organisms with ships' ballast water is recognized as a major pathway of non-indigenous species introduction and addressed in a few recent legislative initiatives. Among other they imply scientific and technical research and monitoring to be conducted in a efficient and reliable way. The recent development of DNA barcoding and metabarcoding technologies opens new opportunities for biodiversity and biosecurity surveillance. In the current study, the performance of metabarcoding approach was assessed in comparison to the conventional (visual) observations, during the en route experimental ballast water survey. Opportunities and limitations of the molecular method were identified from taxonomical datasets rendered by two molecular markers of different degree of universality - the universal cytochrome oxydase sub-unit I gene and a fragment of RuBisCO gene. The cost-efficacy and possible improvements of these methods are discussed for the further successful development and implementation of the approach in ballast water control and NIS surveillance. © 2015 Elsevier Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/86303
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: Klaipeda University, H. Manto 84, Klaipeda, Lithuania; Coastal and Freshwater Group, Cawthron Institute, 98 Halifax Street East, Nelson, New Zealand; Department of Functional Biology, University of Oviedo, C/Julian Claveria s/n, Oviedo, Spain; Biology Department, Marine Biology Section, Ghent University, Krijgslaan, 281/S8, Gent, Belgium; Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, Laboratory Center, Erling Skjalgsons gt. 1, Trondheim, Norway
Recommended Citation:
Zaiko A.,Martinez J.L.,Schmidt-Petersen J.,et al. Metabarcoding approach for the ballast water surveillance - An advantageous solution or an awkward challenge?[J]. Marine Pollution Bulletin,2015-01-01,92(2018-01-02)