DOI: 10.1016/j.marpolbul.2015.10.072
Scopus记录号: 2-s2.0-84951023339
论文题名: Neutral red cytotoxicity assays for assessing in vivo carbon nanotube ecotoxicity in mussels - Comparing microscope and microplate methods
作者: Miller M.A. ; Bankier C. ; Al-Shaeri M.A.M. ; Hartl M.G.J.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2015
卷: 101, 期: 2 起始页码: 903
结束页码: 907
语种: 英语
英文关键词: Carbon nanotubes
; Cytotoxicity
; Ecotoxicity
; Mytilus
; Neutral red
Scopus关键词: Carbon nanotubes
; Cytotoxicity
; Molluscs
; Nanotubes
; Yarn
; Concentration-dependent
; Cytotoxicity assays
; Ecotoxicity
; Labour-intensive
; Mytilus
; Neutral Red
; Retention time
; Sample volume
; Multiwalled carbon nanotubes (MWCN)
; multi walled nanotube
; neutral red
; single walled nanotube
; carbon nanotube
; neutral red
; sea water
; bioaccumulation
; bioassay
; carbon
; ecotoxicology
; environmental impact assessment
; marine pollution
; mollusc
; pollution exposure
; retention
; Article
; concentration (parameters)
; controlled study
; ecotoxicity
; electron microscope
; in vivo study
; lysosome
; microplate reader
; Mytilus
; nonhuman
; room temperature
; animal
; bioassay
; bivalve
; chemistry
; comparative study
; cytology
; devices
; dose response
; drug effects
; ecotoxicology
; environmental monitoring
; hemolymph
; procedures
; sensitivity and specificity
; spectrophotometry
; theoretical model
; transmission electron microscopy
; Mytilus
; Mytilus sp.
; Animals
; Biological Assay
; Bivalvia
; Dose-Response Relationship, Drug
; Ecotoxicology
; Environmental Monitoring
; Hemolymph
; Lysosomes
; Microscopy, Electron, Transmission
; Models, Theoretical
; Nanotubes, Carbon
; Neutral Red
; Seawater
; Sensitivity and Specificity
; Spectrophotometry
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: The purpose of the present study was to compare two neutral red retention methods, the more established but very labour-intensive microscope method (NRR) against the more recently developed microplate method (NRU). The intention was to explore whether the sample volume throughput could be increased and potential operator bias avoided. Mussels Mytilus sp. were exposed in vivo to 50, 250 and 500μgL-1 single (SWCNTs) or multi-walled carbon nanotubes (MWCNTs). Using the NRR method, SWCNTs and MWCNTs caused concentration dependent decreases in neutral red retention time. However, a concentration dependent decrease in optical density was not observed using the NRU method. We conclude that the NRU method is not sensitive enough to assess carbon nanotube ecotoxicity in vivo in environmentally relevant media, and recommend using the NRR method. © 2015 Elsevier Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/86308
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
There are no files associated with this item.
作者单位: Centre for Marine Biodiversity and Biotechnology, School of Life Sciences, Heriot-Watt University, Edinburgh, Scotland, United Kingdom; King Abdulaziz University, Department of Biological Sciences, Faculty of Sciences, Jeddah, Saudi Arabia
Recommended Citation:
Miller M.A.,Bankier C.,Al-Shaeri M.A.M.,et al. Neutral red cytotoxicity assays for assessing in vivo carbon nanotube ecotoxicity in mussels - Comparing microscope and microplate methods[J]. Marine Pollution Bulletin,2015-01-01,101(2)