| DOI: | 10.1016/j.marpolbul.2015.10.064
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| Scopus记录号: | 2-s2.0-84951181637
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| 论文题名: | Occurrence of diverse alkane hydroxylase alkB genes in indigenous oil-degrading bacteria of Baltic Sea surface water |
| 作者: | Viggor S.; Jõesaar M.; Vedler E.; Kiiker R.; Pärnpuu L.; Heinaru A.
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| 刊名: | Marine Pollution Bulletin
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| ISSN: | 0025-326X
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| EISSN: | 1879-3363
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| 出版年: | 2015
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| 卷: | 101, 期:2 | | 起始页码: | 507
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| 结束页码: | 516
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| 语种: | 英语
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| 英文关键词: | Alkane hydroxylase
; AlkB gene
; Baltic Sea
; N-alkane degrading bacterial strains
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| Scopus关键词: | Bacteria
; Crude oil
; Diesel fuels
; Paraffins
; Seawater
; Surface waters
; Alkane hydroxylase
; Bacterial community
; Bacterial strains
; Baltic sea
; Gene clone libraries
; Oil-degrading bacteria
; Reference strains
; Sequence identity
; Genes
; diesel fuel
; heptane
; hexadecane
; petroleum
; surface water
; alkane
; cytochrome P450 4A
; gasoline
; n-hexadecane
; petroleum
; sea water
; bacterium
; gene expression
; marine pollution
; microbial activity
; microcosm
; oil pollution
; species occurrence
; alkB gene
; Article
; bacterial gene
; bacterial growth
; bacterial strain
; bacterium isolate
; bacterium isolation
; carbon source
; controlled study
; gene amplification
; genetic code
; genetic variability
; geographic distribution
; microbial community
; molecular cloning
; nonhuman
; oil spill
; polymerase chain reaction
; sequence analysis
; water sampling
; bacterium
; genetics
; isolation and purification
; metabolism
; microbiology
; phylogeny
; Rhodococcus
; Atlantic Ocean
; Baltic Sea
; Bacteria (microorganisms)
; Limnobacter
; Pedobacter
; Pseudomonas
; Rhodococcus
; Alkanes
; Bacteria
; Cytochrome P-450 CYP4A
; Gasoline
; Genes, Bacterial
; Petroleum
; Phylogeny
; Rhodococcus
; Seawater
|
| Scopus学科分类: | Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
|
| 英文摘要: | Formation of specific oil degrading bacterial communities in diesel fuel, crude oil, heptane and hexadecane supplemented microcosms of the Baltic Sea surface water samples was revealed. The 475 sequences from constructed alkane hydroxylase alkB gene clone libraries were grouped into 30 OPFs. The two largest groups were most similar to Pedobacter sp. (245 from 475) and Limnobacter sp. (112 from 475) alkB gene sequences. From 56 alkane-degrading bacterial strains 41 belonged to the Pseudomonas spp. and 8 to the Rhodococcus spp. having redundant alkB genes. Together 68 alkB gene sequences were identified. These genes grouped into 20 OPFs, half of them being specific only to the isolated strains. Altogether 543 diverse alkB genes were characterized in the brackish Baltic Sea water; some of them representing novel lineages having very low sequence identities with corresponding genes of the reference strains. © 2015 Elsevier Ltd. |
| Citation statistics: |
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| 资源类型: | 期刊论文
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/86386
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| Appears in Collections: | 过去全球变化的重建
全球变化的国际研究计划
|
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作者单位: | Institute of Molecular and Cell Biology, Department of Genetics, University of Tartu, 23 Riia Street, Tartu, Estonia
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| Recommended Citation: | |
Viggor S.,Jõesaar M.,Vedler E.,et al. Occurrence of diverse alkane hydroxylase alkB genes in indigenous oil-degrading bacteria of Baltic Sea surface water[J]. Marine Pollution Bulletin,2015-01-01,101(2)
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