DOI: 10.1016/j.marpolbul.2015.05.012
Scopus记录号: 2-s2.0-84931562090
论文题名: Flow cytometric applicability to evaluate UV inactivation of phytoplankton in marine water samples
作者: Olsen R.O. ; Hess-Erga O.-K. ; Larsen A. ; Thuestad G. ; Tobiesen A. ; Hoell I.A.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2015
卷: 96, 期: 2018-01-02 起始页码: 279
结束页码: 285
语种: 英语
英文关键词: CFDA-AM
; Flow cytometry
; Live/dead detection
; Marine phytoplankton
; Ultraviolet irradiation
; Viability
Scopus关键词: Aquatic organisms
; Disinfection
; Esters
; Flow cytometry
; Irradiation
; Marine biology
; Acetoxymethyl esters
; Colony forming units
; Irradiated samples
; Marine phytoplankton
; Nonindigenous species
; Tetraselmis suecica
; Ultraviolet irradiations
; Viability
; Phytoplankton
; 5 carboxyfluorescein diacetate acetoxymethyl ester
; esterase
; sea water
; unclassified drug
; 5-carboxyfluorescein diacetate acetoxymethyl ester
; carboxyfluoresceindiacetate
; fluorescein derivative
; sea water
; cell organelle
; colony
; flow cytometry
; irradiation
; phytoplankton
; ultraviolet radiation
; viability
; Article
; cell damage
; colony forming unit
; controlled study
; flow cytometry
; measurement accuracy
; nonhuman
; phytoplankton
; plate count
; Tetraselmis suecica
; ultraviolet irradiation
; water analysis
; water sampling
; chemistry
; disinfection
; green alga
; procedures
; ultraviolet radiation
; Tetraselmis suecica
; Chlorophyta
; Disinfection
; Flow Cytometry
; Fluoresceins
; Phytoplankton
; Seawater
; Ultraviolet Rays
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: Disinfection of microbes is of importance to prevent the spread of pathogens and non-indigenous species in the environment. Here we test the applicability of using flow cytometry (FCM) to evaluate inactivation of the phytoplankter Tetraselmis suecica after UV irradiation and labeling with the esterase substrate 5-carboxyfluorescein diacetate acetoxymethyl ester (CFDA-AM). Non-irradiated and UV irradiated samples were analyzed with the plate count technique and FCM for 24. days. The numbers of colony forming units were used as a standard to develop a FCM protocol. Our protocol readily distinguishes live and dead cells, but challenges were encountered when determining whether UV damaged cells are dying or repairable. As damaged cells can represent a risk to aquatic organisms and/or humans, this was taken into account when developing the FCM protocol. In spite of the above mentioned challenges we argue that FCM represents an accurate and rapid method to analyze T. suecica samples. © 2015 The Authors. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/86516
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: Stord/Haugesund University College, Bjoernsonsgt. 45, Haugesund, Norway; Uni Research Environment, Thormoehlensgt. 49 B, Bergen, Norway; Norwegian Institute for Water Research, Thormoehlensgt. 53 D, Bergen, Norway; Norwegian Institute for Water Research, Gaustadalléen 21, Oslo, Norway
Recommended Citation:
Olsen R.O.,Hess-Erga O.-K.,Larsen A.,et al. Flow cytometric applicability to evaluate UV inactivation of phytoplankton in marine water samples[J]. Marine Pollution Bulletin,2015-01-01,96(2018-01-02)