DOI: 10.1016/j.marpolbul.2015.11.016
Scopus记录号: 2-s2.0-84951127776
论文题名: Detection of human-derived fecal contamination in Puerto Rico using carbamazepine, HF183 Bacteroides, and fecal indicator bacteria
作者: Wade C. ; Otero E. ; Poon-Kwong B. ; Rozier R. ; Bachoon D.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2015
卷: 101, 期: 2 起始页码: 872
结束页码: 877
语种: 英语
英文关键词: Carbamazepine
; Caribbean
; Chemical fecal indicators
; E. coli
; Pharmaceutical
; Puerto Rico
Scopus关键词: Amides
; Drug products
; Enzymes
; Escherichia coli
; Indicators (chemical)
; Pollution
; Pollution detection
; Polymerase chain reaction
; River pollution
; Surface waters
; Water analysis
; Carbamazepine
; Caribbean
; E. coli
; Fecal indicators
; Puerto Rico
; Water pollution
; carbamazepine
; carbamazepine
; water pollutant
; biomarker
; concentration (composition)
; drug
; enzyme
; fecal coliform
; feces
; Article
; bacterium detection
; Bacteroides
; biogeographic region
; concentration (parameters)
; controlled study
; enzyme linked immunosorbent assay
; Escherichia coli
; feces
; indicator organism
; limit of detection
; nonhuman
; polymerase chain reaction
; Puerto Rico
; water contamination
; water sampling
; analysis
; Bacteroides
; Caribbean
; environmental monitoring
; Escherichia coli
; feces
; genetics
; human
; microbiology
; procedures
; water pollutant
; Caribbean Islands
; Puerto Rico
; Bacteroides
; Escherichia coli
; Bacteroides
; Carbamazepine
; Caribbean Region
; Environmental Monitoring
; Enzyme-Linked Immunosorbent Assay
; Escherichia coli
; Feces
; Humans
; Puerto Rico
; Water Microbiology
; Water Pollutants, Chemical
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: The level of fecal pollution in 17 sites in Puerto Rico was determined by Escherichia coli (E. coli) enumeration using an enzyme substrate medium and Quanti-Tray®/2000. Human fecal pollution was identified using an enzyme-linked immunosorbent assay for the detection of carbamazepine (CBZ) and quantitative polymerase chain reaction (qPCR) detection of the human Bacteroides marker, HF183. Carbamazepine was detected in 16 out of 17 sites, including Condado Lagoon, a popular recreational area. Elevated E. coli levels (>410CFU100mL-1) were detected in 13 sites. Average CBZ concentrations ranged from 0.005μgL-1 to 0.482μgL-1 and 7 sites were positive for HF183. Higher CBZ concentrations were associated with the detection of HF183 (Mann-Whitney test; U=42.0; df=7; 1-tailed P value=0.013). This was the second study to determine surface water concentrations of CBZ in the Caribbean and the first in Puerto Rico. © 2015 Elsevier Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/86530
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: Department of Marine Sciences, University of Puerto Rico, Mayaguez Campus, P.O. Box 9013, Mayaguez, PR, United States; Department of Biological and Environmental Sciences, Georgia College and State University, Campus Box 81, Milledgeville, GA, United States
Recommended Citation:
Wade C.,Otero E.,Poon-Kwong B.,et al. Detection of human-derived fecal contamination in Puerto Rico using carbamazepine, HF183 Bacteroides, and fecal indicator bacteria[J]. Marine Pollution Bulletin,2015-01-01,101(2)