Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要:
Estuaries are very important ecosystems with great ecological and economic value, but usually highly impacted by anthropogenic pressure. Thus, the assessment of pollution levels in these habitats is critical in order to evaluate their environmental quality. In this work, we combined complementary metallomic and proteomic approaches with the aim to monitor the effects of environmental pollution on Scrobicularia plana clams captured in three estuarine systems from the south coast of Portugal; Arade estuary, Ria Formosa and Guadiana estuary. Multi-elemental profiling of digestive glands was carried out to evaluate the differential pollution levels in the three study areas. Then, proteomic analysis by means of two-dimensional gel electrophoresis and mass spectrometry revealed twenty-one differential proteins, which could be associated with multiple toxicological mechanisms induced in environmentally stressed organisms. Accordingly, it could be concluded that the combination of different omic approaches presents a great potential in environmental research. � 2016 Elsevier Ltd
Department of Chemistry, Faculty of Experimental Sciences, University of Huelva, Campus de El Carmen, Huelva, Spain; Campus of Excellence International ceiA3, University of Huelva, Spain; Research Center of Health and Environment (CYSMA), University of Huelva, Campus de El Carmen, Huelva, Spain; UCIBIO-REQUIMTE, Chemistry Department, Faculty of Sciences and Technology, Universidade NOVA de Lisboa, Caparica, Portugal; ProteoMass Scientific Society, MadanPark, Rua dos Inventores s/n, Monte de Caparica, Caparica, Portugal; CIMA, Faculty of Marine and Environmental Sciences, University of Algarve, Campus de Gambelas, Faro, Portugal
Recommended Citation:
Gonz�lez-Dom�nguez R.,Santos H.M.,Bebianno M.J.,et al. Combined proteomic and metallomic analyses in Scrobicularia plana clams to assess environmental pollution of estuarine ecosystems[J]. Marine Pollution Bulletin,2016-01-01,113(2018-01-02)