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DOI: 10.1016/j.marpolbul.2016.10.049
Scopus记录号: 2-s2.0-85003977149
论文题名:
Identification and quantification of microplastics using Nile Red staining
作者: Shim W.J.; Song Y.K.; Hong S.H.; Jang M.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2016
卷: 113, 期:2018-01-02
起始页码: 469
结束页码: 476
语种: 英语
英文关键词: identification ; microplastics ; Nile Red ; staining
Scopus关键词: Ethylene ; Fluorescence ; Hexane ; Identification (control systems) ; Polyethylenes ; Thermoplastic elastomers ; Fluorescent dyes ; Green fluorescence ; Microplastics ; Nile red ; Poly(ethylene vinyl acetate) ; Polymer particles ; staining ; Weathering tests ; Polypropylenes ; ethylene vinyl acetate copolymer ; fluorescent dye ; hexane ; microplastic ; nile red ; plastic ; polyamide ; polycarbonate ; polyester ; polyethylene ; polypropylene ; polystyrene ; polyurethan ; polyvinylchloride ; unclassified drug ; fluorescent dye ; nile red ; oxazine derivative ; water pollutant ; dye ; identification method ; marine pollution ; particle size ; plastic ; pollution monitoring ; polymer ; quantitative analysis ; Article ; controlled study ; fluorescence ; product recovery ; quantitative analysis ; sand ; weathering ; analysis ; infrared spectroscopy ; staining ; water pollutant ; Fluorescent Dyes ; Oxazines ; Plastics ; Polyethylene ; Spectroscopy, Fourier Transform Infrared ; Staining and Labeling ; Water Pollutants, Chemical
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science ; Earth and Planetary Sciences: Oceanography ; Environmental Science: Pollution
英文摘要: We investigated the applicability of Nile Red (NR), a fluorescent dye, for microplastic analysis, and determined the optimal staining conditions. Five mg/L NR solution in n-hexane effectively stained plastics, and they were easily recognized in green fluorescence. The NR staining method was successfully applied to micro-sized polyethylene, polypropylene, polystyrene, polycarbonate, polyurethane, and poly(ethylene-vinyl acetate), except for polyvinylchloride, polyamide and polyester. The recovery rate of polyethylene (100–300 μm) spiked to pretreated natural sand was 98% in the NR stating method, which was not significantly (p < 0.05) different with FT-IR identification. The NR staining method was suitable for discriminating fragmented polypropylene particles from large numbers of sand particles in laboratory weathering test samples. The method is straightforward and quick for identifying and quantifying polymer particles in the laboratory controlled samples. Further studies, however, are necessary to investigate the application of NR staining to field samples with organic remnants. © 2016 Elsevier Ltd
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/86644
Appears in Collections:过去全球变化的重建
全球变化的国际研究计划

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作者单位: Oil and POPs Research Laboratory, Korea Institute of Ocean Science and Technology, 41 Jangmok-1-gil, Geoje, South Korea; Department of Marine Environmental Sciences, Korea University of Science and Technology, Gajeong-ro, Daejeon, South Korea

Recommended Citation:
Shim W.J.,Song Y.K.,Hong S.H.,et al. Identification and quantification of microplastics using Nile Red staining[J]. Marine Pollution Bulletin,2016-01-01,113(2018-01-02)
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