DOI: 10.1016/j.marpolbul.2016.06.038
Scopus记录号: 2-s2.0-84991007379
论文题名: In situ detection of alkB2 gene involved in Alcanivorax borkumensis SK2Thydrocarbon biodegradation
作者: Matturro B. ; Frascadore E. ; Cappello S. ; Genovese M. ; Rossetti S.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2016
卷: 110, 期: 1 起始页码: 378
结束页码: 382
语种: 英语
英文关键词: Alcanivorax borkumensis SK2T
; alkB2 gene
; GeneCARD-FISH
; n-Alkanes
; Petroleum-polluted seawater
Scopus关键词: alkane
; bacterial protein
; cytochrome P450 4A
; hydrocarbon
; petroleum
; sea water
; water pollutant
; Alcanivoraxaceae
; bioremediation
; fluorescence in situ hybridization
; genetics
; Italy
; metabolism
; microbiology
; reproducibility
; water pollutant
; Alcanivoraceae
; Alkanes
; Bacterial Proteins
; Biodegradation, Environmental
; Cytochrome P-450 CYP4A
; Hydrocarbons
; In Situ Hybridization, Fluorescence
; Italy
; Petroleum
; Reproducibility of Results
; Seawater
; Water Pollutants, Chemical
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: This study aimed to develop a new assay based on the whole cell hybridization in order to monitor alkane hydroxylase genes (alkB system) of the marine bacterium Alcanivorax borkumensis SK2Tcommonly reported as the predominant microorganism responsible for the biodegradation of n-alkanes which are the major fraction of petroleum hydrocarbons. The assay based on the whole cell hybridization targeting alkB2 gene was successfully developed and calibrated on a pure culture of Alcanivorax borkumensis SK2Twith a detection efficiency up to 80%. The approach was further successfully validated on hydrocarbon-contaminated seawater and provided cells abundance (6.74E + 04 alkB2-carrying cells mL− 1) higher of about one order of magnitude than those obtained by qPCR (4.96E + 03 alkB2 gene copies mL− 1). This study highlights the validity of the assay for the detection at single cell level of key-functional genes involved in the biodegradation of n-alkanes. © 2016 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/86811
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: Water Research Institute, IRSA-CNR, Via Salaria km 29,300, Monterotondo, RM, Italy; Institute of Marine and Coastal Environments, IAMC-CNR, Spianata S. Raineri, 86, Messina, ME, Italy
Recommended Citation:
Matturro B.,Frascadore E.,Cappello S.,et al. In situ detection of alkB2 gene involved in Alcanivorax borkumensis SK2Thydrocarbon biodegradation[J]. Marine Pollution Bulletin,2016-01-01,110(1)