DOI: 10.1016/j.marpolbul.2016.06.031
Scopus记录号: 2-s2.0-84990922453
论文题名: Optimisation for assay of fluorescein diacetate hydrolytic activity as a sensitive tool to evaluate impacts of pollutants and nutrients on microbial activity in coastal sediments
作者: Jiang S. ; Huang J. ; Lu H. ; Liu J. ; Yan C.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2016
卷: 110, 期: 1 起始页码: 424
结束页码: 431
语种: 英语
英文关键词: Coastal sediment
; Enzyme assay
; Fluorescein diacetate
; Microbial activity
; Optimisation
Scopus关键词: fluorescein derivative
; fluorescein diacetate
; water pollutant
; analysis
; chemistry
; China
; environment
; hydrolysis
; metabolism
; microbiological examination
; microbiology
; pH
; procedures
; reproducibility
; sediment
; sensitivity and specificity
; water pollutant
; China
; Environment
; Fluoresceins
; Geologic Sediments
; Hydrogen-Ion Concentration
; Hydrolysis
; Microbiological Techniques
; Reproducibility of Results
; Sensitivity and Specificity
; Water Pollutants, Chemical
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: Fluorescein diacetate (FDA) assay has been widely applied in coastal research to quantify microbial activity in sediments. However, the present FDA assay procedures embodied in sediment studies potentially include operational errors since the protocol was established for studies of terrestrial soil. In the present study, we optimised the procedure of FDA assay using sandy and cohesive sediments to improve experiential sensitivity and reproducibility. The optimised method describes quantitative measurement of the fluorescein produced when 1.0 g of fresh sediment is incubated with 50 mM phosphate buffer solution (pH: 7.3) and glass beads (2 g) at 35 °C for 1 h under a rotation of 50 rpm. The covariation coefficient of the optimised method ranged from 1.9% to 3.8% and the method sensitivity ranged from 0.25 to 1.57. The improved protocol provides a more reliable measurement of the FDA hydrolysis rate over a wide range of sediments compared to the original method. © 2016 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/86838
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, Xiamen University, China; Geography Department, Museum Building, School of Natural Sciences, Trinity College, University of Dublin, Ireland
Recommended Citation:
Jiang S.,Huang J.,Lu H.,et al. Optimisation for assay of fluorescein diacetate hydrolytic activity as a sensitive tool to evaluate impacts of pollutants and nutrients on microbial activity in coastal sediments[J]. Marine Pollution Bulletin,2016-01-01,110(1)