BfpA protein, E coli
; Escherichia coli protein
; fimbria protein
; sewage
; verotoxin 1
; analysis
; Brazil
; environmental exposure
; Escherichia coli
; genetics
; human
; isolation and purification
; microbiology
; multiplex polymerase chain reaction
; pathogenicity
; procedures
; recreation
; risk assessment
; sewage
; swimming
; United States
; water quality
; Bathing Beaches
; Brazil
; Environmental Exposure
; Escherichia coli
; Escherichia coli Proteins
; Fimbriae Proteins
; Humans
; Multiplex Polymerase Chain Reaction
; Recreation
; Risk Assessment
; Sewage
; Shiga Toxin 1
; United States
; Waste Disposal, Fluid
; Water Microbiology
; Water Quality
Scopus学科分类:
Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要:
Marine beaches are important recreational and economic resources in Brazil, but the beaches& water quality is negatively impacted by the discharge of domestic sewage effluent. The occurrence of diarrheagenic Escherichia coli among the E. coli isolated from three Brazilian marine beaches was investigated. Multiplex and single step PCR were used to screen 99 E. coli isolates for ten target toxin genes. Six toxin genes, stx1, eae, estp, esth, astA, and bfpA, were identified in 1% to 35% of the isolates. A quantitative microbial risk assessment (QMRA) of human exposure to diarrheagenic E. coli during marine recreation was carried out. The results indicated that the diarrheagenic E. coli risk is well below the U.S. EPA's recommended daily recreational risk benchmark. However, the overall recreational health risk due to all pathogens in the water could be much higher and exceeded the U.S. EPA's benchmark. � 2016 Elsevier Ltd
Biomedical Science Institute, Microbiology Department, University of S�o Paulo, Estado de S�o PauloSP, Brazil; Department of Civil and Environmental Engineering, University of California, Irvine, CA, United States
Recommended Citation:
Rodrigues V.F.V.,Rivera I.N.G.,Lim K.-Y.,et al. Detection and risk assessment of diarrheagenic E. coli in recreational beaches of Brazil[J]. Marine Pollution Bulletin,2016-01-01,109(1)