Transcription of a novel P450 gene varies with some factors (pollutant exposure, temperature, time, and body region) in a marine oligochaete (Thalassodrilides sp.)
cytochrome P450
; polycyclic aromatic hydrocarbon
; water pollutant
; analysis
; animal
; environmental monitoring
; genetics
; metabolism
; Oligochaeta
; physiological stress
; physiology
; sediment
; temperature
; toxicity
; water pollutant
; Animals
; Cytochrome P-450 Enzyme System
; Environmental Monitoring
; Geologic Sediments
; Oligochaeta
; Polycyclic Aromatic Hydrocarbons
; Stress, Physiological
; Temperature
; Water Pollutants, Chemical
Scopus学科分类:
Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要:
Cytochrome P450 (CYP) enzymes play important roles in the metabolism of exogenous compounds such as polycyclic aromatic hydrocarbons (PAHs). A novel, full-length CYP gene (CYP4V30) was identified in the oligochaete Thalassodrilides sp. CYP4V30 mRNA expression was studied in worms exposed to PAH-polluted (Σ16PAHs; 37441 ng/g dry weight) or unpolluted (Σ16PAHs; 19 ng/g dry weight) sediment. CYP4V30 expression was much higher in worms exposed to contaminated sediments than in those exposed to unpolluted sediments at some temperatures (20 and 25 �C) and exposure durations (11-fold increase at 20 �C, 10-day exposure), but not at 15 �C or other exposure durations (P < 0.05). CYP4V30 mRNA expression was higher in the middle of the body than in the posterior (P < 0.05). The variation in transcriptional response with exposure time, temperature, and body region indicates that these factors should be considered when monitoring marine sediment pollution. � 2016 Elsevier Ltd
National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, Maruishi 2-17-5, Hatsukaichi, Hiroshima, Japan; South Ehime Fisheries Research Center, Ehime University, 1289-1, Funakoshi, Ainan, Ehime, Japan
Recommended Citation:
Ito M.,Ito K.,Ohta K.,et al. Transcription of a novel P450 gene varies with some factors (pollutant exposure, temperature, time, and body region) in a marine oligochaete (Thalassodrilides sp.)[J]. Marine Pollution Bulletin,2016-01-01,109(1)