DOI: 10.1016/j.marpolbul.2016.04.060
Scopus记录号: 2-s2.0-84966800352
论文题名: Absence of selenium protection against methylmercury toxicity in harbour seal leucocytes in vitro
作者: Das K. ; Dupont A. ; De Pauw-Gillet M.C. ; Debier C. ; Siebert U.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2016
卷: 108, 期: 2018-01-02 起始页码: 70
结束页码: 76
语种: 英语
英文关键词: Harbour seal
; In vitro approach
; Lymphocytes
; Marine mammals
; Methylmercury
; Phoca vitulina
; Selenium
Scopus关键词: Cell membranes
; Cell proliferation
; Mammals
; Mitochondria
; Selenium
; Selenium compounds
; Sodium
; T-cells
; Toxicity
; Harbour seal
; In-vitro
; Marine mammals
; Methyl mercury
; Phoca vitulina
; Lymphocytes
; DNA
; methylmercury
; selenite
; selenium
; selenomethionine
; sodium selenite
; mercury
; methylmercury derivative
; selenium
; water pollutant
; immune system
; inhibition
; methylmercury
; pinniped
; selenium
; toxicity
; Article
; cell proliferation
; concentration (parameters)
; controlled study
; DNA synthesis
; heavy metal poisoning
; immunocompetent cell
; in vitro study
; leukocyte
; lymphocyte
; lymphocyte proliferation
; mitochondrial membrane potential
; nonhuman
; North Sea
; Pinnipedia
; T lymphocyte
; animal
; cell culture
; chemistry
; drug effects
; leukocyte
; Phoca
; toxicity
; water pollutant
; Atlantic Ocean
; North Sea
; Mammalia
; Phoca vitulina
; Animals
; Cell Proliferation
; Cells, Cultured
; Leukocytes
; Membrane Potential, Mitochondrial
; Mercury
; Methylmercury Compounds
; Phoca
; Selenium
; Water Pollutants, Chemical
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: Previous studies described high concentrations of mercury (Hg) and selenium (Se) in the blood of harbour seals, Phoca vitulina from the North Sea. In the present study, we evaluated the in vitro potential protective effects of sodium selenite (Na2SeO3) and selenomethionine (SeMet) on cell proliferation of harbour seal lymphocytes exposed to MeHgCl 0.75 μM. In vitro exposure of ConA-stimulated T lymphocytes resulted in severe inhibition of DNA synthesis, likely linked to severe loss of mitochondrial membrane potential at 0.75 μM. Neither selenite nor SeMet showed a protective effect against MeHg toxicity expressed at the T lymphocyte proliferation level for harbour seals. Selenite and SeMet did not show negative effects regarding lymphocyte proliferation and mitochondrial membrane potential.To conclude, our results clearly demonstrated that MeHg affected in vitro immune cells exposure with no protective effects of selenium at a molar ratio Hg:Se of 1:10 in harbour seals from the North Sea. © 2016 Elsevier Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/86972
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: Laboratory of Oceanology, MARE Center, University of Liège, B6c, Allée du 6 AoÛt 15, Liège, Belgium; Laboratory of Mammalian Cell Culture (GIGA-R), University of Liège, B6c, Allée du 6 AoÛt 15, Liège, Belgium; Institute of Life Sciences, Université catholique de Louvain, Croix du Sud 2/L7.05.08, Louvain-la-Neuve, Belgium; Institute for Terrestrial and Aquatic Wildlife Research, University of Veterinary Medicine Hannover, Foundation, Werftstrasse 6, Buesum, Germany
Recommended Citation:
Das K.,Dupont A.,De Pauw-Gillet M.C.,et al. Absence of selenium protection against methylmercury toxicity in harbour seal leucocytes in vitro[J]. Marine Pollution Bulletin,2016-01-01,108(2018-01-02)