DOI: 10.1016/j.marpolbul.2016.04.011
Scopus记录号: 2-s2.0-84992311369
论文题名: Bioaugmentation strategy employing a microbial consortium immobilized in chitosan beads for oil degradation in mesocosm scale
作者: Dellagnezze B.M. ; Vasconcellos S.P. ; Angelim A.L. ; Melo V.M.M. ; Santisi S. ; Cappello S. ; Oliveira V.M.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2016
卷: 107, 期: 1 起始页码: 107
结束页码: 117
语种: 英语
英文关键词: Bioaugmentation
; Consortium
; Mesocosm
; Metagenomic clones
; Petroleum
Scopus关键词: Aromatic compounds
; Bacteria
; Bacteriology
; Biochemical oxygen demand
; Biodegradation
; Chemical analysis
; Chitin
; Chitosan
; Cloning
; Crude oil
; Hydrocarbons
; Paraffins
; Petroleum reservoir engineering
; Petroleum reservoirs
; RNA
; Bacillus subtilis strains
; Bio-augmentation
; Biological oxygen demand
; Consortium
; Hydrocarbon degradation
; Mesocosms
; Metagenomic clones
; Microbiological analysis
; Aromatic hydrocarbons
; alkane
; aromatic compound
; chitosan
; chitosan bead
; hydrocarbon
; petroleum
; RNA 16S
; unclassified drug
; chitosan
; petroleum
; RNA 16S
; aromatic hydrocarbon
; biodegradation
; clone
; hydrocarbon reservoir
; mesocosm
; microorganism
; polysaccharide
; Article
; bacterial strain
; bacterium identification
; bioaugmentation
; biochemical oxygen demand
; bioremediation
; chemical analysis
; colony forming unit
; concentration (parameters)
; controlled study
; environmental factor
; mesocosm
; microbial consortium
; nonhuman
; population abundance
; RNA sequence
; sequence analysis
; species diversity
; waste management
; water analysis
; water pollution
; bacterium
; bioremediation
; genetics
; immobilized cell
; metabolism
; Bacillus subtilis
; Bacteria (microorganisms)
; Bacteria
; Biodegradation, Environmental
; Cells, Immobilized
; Chitosan
; Hydrocarbons
; Microbial Consortia
; Petroleum
; RNA, Ribosomal, 16S
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: A bacterial consortium composed by four metagenomic clones and Bacillus subtilis strain CBMAI 707, all derived from petroleum reservoirs, was entrapped in chitosan beads and evaluated regarding hydrocarbon degradation capability. Experiments were carried out in mesocosm scale (3000 L) with seawater artificially polluted with crude oil. At different time intervals, mesocosms were sampled and subjected to GC-FID and microbiological analyses, as total and heterotrophic culturable bacterial abundance (DAPI and CFU count), biological oxygen demand (BOD) and taxonomic diversity (massive sequencing of 16S rRNA genes). The results obtained showed that degradation of n-alkane hydrocarbons was similar between both treatments. However, aromatic compound degradation was more efficient in bioaugmentation treatment, with biodegradation percentages reaching up to 99% in 30 days. Community dynamics was different between treatments and the consortium used in the bioaugmentation treatment contributed to a significant increase in aromatic hydrocarbon degradation. © 2016 Elsevier Ltd. All rights reserved. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/87027
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: Division of Microbial Resources, Research Center for Chemistry, Biology and Agriculture (CPQBA), Campinas University - UNICAMP, CP 6171, Campinas, SP, Brazil; Federal University of São Paulo (UNIFESP), Rua Prof. Artur Riedel, 275, Jd. Eldorado, Diadema, SP, Brazil; Lembiotech (UFC), Federal University of Ceará, Campus Do Pici, Av. Humberto Monte, 2977, Bloco 909, Fortaleza, CE, Brazil; Institute for Coastal Marine Environment (IAMC), Consiglio Nazionale Delle Ricerche (CNR) of Messina, Messina, Italy
Recommended Citation:
Dellagnezze B.M.,Vasconcellos S.P.,Angelim A.L.,et al. Bioaugmentation strategy employing a microbial consortium immobilized in chitosan beads for oil degradation in mesocosm scale[J]. Marine Pollution Bulletin,2016-01-01,107(1)