DOI: 10.1016/j.marpolbul.2016.02.041
Scopus记录号: 2-s2.0-84959048735
论文题名: Biodegradation potentiality of psychrophilic bacterial strain Oleispira antarctica RB-8T
作者: Gentile G. ; Bonsignore M. ; Santisi S. ; Catalfamo M. ; Giuliano L. ; Genovese L. ; Yakimov M.M. ; Denaro R. ; Genovese M. ; Cappello S.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2016
卷: 105, 期: 1 起始页码: 125
结束页码: 130
语种: 英语
英文关键词: Bioremediation
; Hydrocarbonoclastic bacteria
; Oil spill
; Oleispira antarctica
; Psychrophilic bacteria
Scopus关键词: Bacteria
; Biodegradation
; Bioremediation
; Chromatography
; Crude oil
; Degradation
; Fighter aircraft
; Gas chromatography
; Gas generators
; Ionization of gases
; Oil spills
; Antarctica
; Bacterial abundance
; Gas chromatography-flame ionization detections
; Hydrocarbon degradation
; Hydrocarbon mixture
; Hydrocarbon sources
; Incubation periods
; Psychrophilic bacteria
; Hydrocarbons
; 4',6 diamidino 2 phenylindole
; fuel
; hydrocarbon
; petroleum
; bacterium
; biodegradation
; bioremediation
; DNA
; growth response
; marine pollution
; oil spill
; pollution effect
; Article
; bacterium culture
; bioremediation
; concentration (parameters)
; controlled study
; DNA determination
; DNA extraction
; flame ionization detection
; gas chromatography
; nonhuman
; Oleispira antarctica
; population abundance
; psychrophilic bacterium
; temperature sensitivity
; water temperature
; Bacteria (microorganisms)
; Oleispira antarctica
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: The present study is focused on assessing the growth and hydrocarbon-degrading capability of the psychrophilic strain Oleispira antarctica RB-8T. This study considered six hydrocarbon mixtures that were tested for 22 days at two different cultivation temperatures (4 and 15 °C). During the incubation period, six sub-aliquots of each culture at different times were processed for total bacterial abundance and GC-FID (gas chromatography-flame ionization detection) hydrocarbon analysis.Results from DNA extraction and DAPI (4',6-diamidino-2-phenylindole) staining showed a linear increase during the first 18 days of the experiment in almost all the substrates used; both techniques showed a good match, but the difference in values obtained was approximately one order of magnitude. GC-FID results revealed a substantial hydrocarbon degradation rate in almost all hydrocarbon sources and in particular at 15 °C rather than 4 °C (for commercial oil engine, oily waste, fuel jet, and crude oil). A more efficient degradation was observed in cultures grown with diesel and bilge water at 4 °C. © 2016 Elsevier Ltd.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/87166
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: Institute for Coastal Marine Environment (IAMC)-CNR of Messina, Messina, Italy; Ph.D School in Biology and Cellular Biotechnology of Univ. of Messina, Messina, Italy; Institute for Coastal Marine Environment (IAMC)-CNR of Napoli, Naples, Italy
Recommended Citation:
Gentile G.,Bonsignore M.,Santisi S.,et al. Biodegradation potentiality of psychrophilic bacterial strain Oleispira antarctica RB-8T[J]. Marine Pollution Bulletin,2016-01-01,105(1)