globalchange  > 过去全球变化的重建
DOI: 10.1016/j.marpolbul.2015.12.010
Scopus记录号: 2-s2.0-84961286550
论文题名:
Esterase activity (EA), total oxidant status (TOS) and total antioxidant capacity (TAC) in gills of Mytilus galloprovincialis exposed to pollutants: Analytical validation and effects evaluation by single and mixed heavy metal exposure
作者: Franco L.; Romero D.; García-Navarro J.A.; Teles M.; Tvarijonaviciute A.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2016
卷: 102, 期:1
起始页码: 30
结束页码: 35
语种: 英语
英文关键词: Cadmium ; Copper ; Lead ; Mussels ; Oxidative stress ; Ternary mixture
Scopus关键词: Antioxidants ; Cadmium ; Centrifugation ; Copper ; Enzymes ; Heavy metals ; Lead ; Mixtures ; Molluscs ; Oxidants ; Oxidative stress ; Pollution ; Analytical validation ; Coefficients of variations ; Esterase activities ; Mussels ; Mytilus galloprovincialis ; Ternary mixtures ; Total antioxidant capacities (TAC) ; Ultracentrifugation ; Esters ; buffer ; cadmium ; copper ; esterase ; heavy metal ; lead ; antioxidant ; biological marker ; esterase ; heavy metal ; oxidizing agent ; water pollutant ; antioxidant ; biomarker ; cadmium ; copper ; lead ; mollusc ; oxidant ; pollution exposure ; analytical parameters ; animal experiment ; Article ; biological monitoring ; concentration (parameters) ; controlled study ; enzyme activity ; gill ; Mytilus galloprovincialis ; nonhuman ; process optimization ; total antioxidant capacity ; total oxidant status ; ultracentrifugation ; validation study ; analysis ; animal ; drug effects ; environmental monitoring ; metabolism ; Mytilus ; procedures ; toxicity ; water pollutant ; Mytilus galloprovincialis ; Toscana virus ; Animals ; Antioxidants ; Biomarkers ; Environmental Monitoring ; Esterases ; Gills ; Metals, Heavy ; Mytilus ; Oxidants ; Water Pollutants, Chemical
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science ; Earth and Planetary Sciences: Oceanography ; Environmental Science: Pollution
英文摘要: The aims of the present study were to optimize and validate methods for esterase activity (EA), total oxidant status (TOS) and total antioxidant capacity (TAC) determination in mussel' gills, and to establish the relationships between these biomarkers and Pb, Cd and Cu pollution, in single form and ternary mixture. Two different buffers for sample homogenization, the need of ultracentrifugation, and analytical validation were evaluated. Coefficients of variation, when buffer without additives and ultracentrifugation were used, were <. 15%, and recovery were 97%-109% in all cases. The EA response tends to decrease with treatments, TOS decreased significantly in Cd and ternary groups, while TAC tended to increase in treatments with Pb, Cd and ternary groups. In conclusion, the methods for EA, TOS and TAC measurements in gills of mussel were precise and accurate and could be interesting resources in biomonitoring programmes. © 2015 Elsevier Ltd.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/87283
Appears in Collections:过去全球变化的重建
全球变化的国际研究计划

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作者单位: Área de Toxicología, Facultad de Veterinaria, Universidad de Murcia, Campus Regional de Excelencia Internacional, Campus Mare Nostrum, Espinardo, Murcia, Spain; Departament de Biologia Cellular, Fisiologia i Immunologia, Universidad Autónoma de Barcelona, Campus d'excellència UAB-CEI, Bellaterra, Barcelona, Spain; Departament de Medicina i Cirugia Animals, Universidad Autónoma de Barcelona, Campus d'excellència UAB-CEI, Bellaterra, Barcelona, Spain

Recommended Citation:
Franco L.,Romero D.,García-Navarro J.A.,et al. Esterase activity (EA), total oxidant status (TOS) and total antioxidant capacity (TAC) in gills of Mytilus galloprovincialis exposed to pollutants: Analytical validation and effects evaluation by single and mixed heavy metal exposure[J]. Marine Pollution Bulletin,2016-01-01,102(1)
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