DOI: 10.1016/j.marpolbul.2017.04.052
Scopus记录号: 2-s2.0-85018971162
论文题名: Hepatocellular adenoma in a European flatfish (Limanda limanda): Genetic alterations in laser-capture micro-dissected tissue and global transcriptomic approach
作者: Lerebours A. ; Chapman E. ; Lyons B.P. ; Bignell J.P. ; Stentiford G.D. ; Rotchell J.M.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2017
卷: 119, 期: 2 起始页码: 120
结束页码: 127
语种: 英语
英文关键词: Contaminants
; Fish
; Genetic changes
; Liver cancer
; retinoblastoma gene
Scopus关键词: Cell signaling
; Dissection
; Fish
; Gene expression
; Genes
; Impurities
; Mammals
; Marine pollution
; Proteins
; Rubidium
; Tumors
; Differentially expressed gene
; Domain-containing proteins
; Genetic changes
; Hepatocellular adenoma
; Liver cancers
; Protein degradation
; Retinoblastoma
; Suppression subtractive hybridization
; Gene expression regulation
; cyclin dependent kinase inhibitor 1B
; Rac1 protein
; biological development
; biomonitoring
; cancer
; flatfish
; gene expression
; genetic analysis
; histopathology
; hybridization
; pollution effect
; tumor
; animal tissue
; ankyrin repeat sterile alpha motif domain containing protein 1B like gene
; arrestin domain containing 3 gene
; Article
; CDKN1B gene
; controlled study
; female
; flatfish
; gene
; gene expression
; gene mutation
; genetic heterogeneity
; laser capture microdissection
; Limanda limanda
; liver adenoma
; liver carcinogenesis
; male
; nonhuman
; North Sea
; oncogene c fos
; Rac 1 gene
; RhoA like gene
; suppression subtractive hybridization
; transcriptomics
; tribbles gene
; tumor suppressor gene
; upregulation
; animal
; flounder
; gene expression profiling
; gene expression regulation
; genetics
; liver adenoma
; liver tumor
; pollutant
; signal transduction
; toxicity
; veterinary
; Atlantic Ocean
; North Sea
; Limanda limanda
; Mammalia
; Pleuronectiformes
; Adenoma, Liver Cell
; Animals
; Environmental Pollutants
; Flounder
; Gene Expression Profiling
; Gene Expression Regulation
; Liver Neoplasms
; North Sea
; Signal Transduction
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: Liver tumours in flatfish have been diagnosed using histopathology for decades to monitor the impacts of marine pollution. Here we describe the application of specific gene (retinoblastoma, Rb) profiling in laser capture micro-dissected samples, and a suppression subtractive hybridization (SSH) approach to isolate differentially expressed genes in hepatocellular adenoma (HCA) samples from dab, Limanda limanda. The Rb profiles from apparently normal and HCA micro-dissected samples of fish from the North Sea showed no significant difference, and genotypic heterogeneity within defined histological phenotypes was observed. In the SSH, sequences associated with cell signalling, cell cycle, gene expression regulation, protein transport and protein degradation were isolated. These included up-regulation of arrestin domain containing 3 (arrdc3), Rac-1 and tribbles, and down-regulation of ankyrin repeat/sterile alpha-motif domain-containing protein 1B-like (ANKS1B-like), c-fos, CDKN1B and RhoA-like sequences, previously implicated in mammalian HCA. This study offers new candidates involved in fish liver tumour development. © 2017 Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/87566
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: School of Environmental Sciences, University of Hull, Cottingham Road, Hull, United Kingdom; Centre for Environment, Fisheries and Aquaculture Science, Weymouth Laboratory, Barrack Road, The Nothe, Weymouth, Dorset, United Kingdom
Recommended Citation:
Lerebours A.,Chapman E.,Lyons B.P.,et al. Hepatocellular adenoma in a European flatfish (Limanda limanda): Genetic alterations in laser-capture micro-dissected tissue and global transcriptomic approach[J]. Marine Pollution Bulletin,2017-01-01,119(2)