globalchange  > 过去全球变化的重建
DOI: 10.1016/j.marpolbul.2017.03.005
Scopus记录号: 2-s2.0-85015719339
论文题名:
Cryptosporidium species from common edible bivalves in Manila Bay, Philippines
作者: Pagoso E.J.A.; Rivera W.L.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2017
卷: 119, 期:1
起始页码: 31
结束页码: 39
语种: 英语
英文关键词: Bivalves ; Cryptosporidium ; Genotype ; Manila Bay ; Oocyst ; Philippines
Scopus关键词: Flotation ; Genes ; RNA ; Bivalves ; Cryptosporidium ; Genotype ; Manila Bay ; Oocyst ; Philippines ; Molluscs ; RNA 18S ; sucrose ; protozoal DNA ; bivalve ; genetic analysis ; genotype ; parasite ; pathogen ; pollution effect ; protein ; 18S rRNA gene ; animal tissue ; Argopecten irradians ; Article ; bay ; bivalve ; controlled study ; Crassostrea ; Crassostrea iredalei ; Cryptosporidium ; Cryptosporidium hominis ; Cryptosporidium meleagridis ; Cryptosporidium parvum ; direct fluorescent antibody technique ; DNA sequence ; flotation ; food contamination ; genotype ; immunomagnetic separation ; Manila Bay ; molecular weight ; mussel ; nonhuman ; nucleotide sequence ; oocyst ; parasite isolation ; Perna viridis ; Philippines ; phylogeny ; polymerase chain reaction ; Ruditapes philippinarum ; animal ; bivalve ; Cryptosporidium ; food contamination ; human ; isolation and purification ; microbiology ; Luzon ; Manila Bay ; Philippines ; Aves ; Bivalvia ; Cryptosporidium ; Cryptosporidium hominis ; Cryptosporidium meleagridis ; Cryptosporidium parvum ; Protozoa ; Animals ; Bays ; Bivalvia ; Cryptosporidium ; DNA, Protozoan ; Food Contamination ; Genotype ; Humans ; Philippines ; Polymerase Chain Reaction
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science ; Earth and Planetary Sciences: Oceanography ; Environmental Science: Pollution
英文摘要: Manila Bay is one of the major propagation sites of edible bivalves in the Philippines. Studies have shown that bivalves might be contaminated with human pathogens like the protozoan parasite Cryptosporidium, one of the major causes of gastroenteritis in the world. In this study, Cryptosporidium from four species of edible bivalves were isolated using a combination of sucrose flotation and immunomagnetic separation. Using direct fluorescent antibody test, Cryptosporidium oocysts were found in 67 out of 144 samples collected. DNA sequence analysis of the 18S rRNA gene of the isolates detected C. parvum and C. hominis (major causes of human cryptosporidiosis) and C. meleagridis (causes infection in avian species). Analysis of the 60 kDa glycoprotein gene further confirmed the genotypes of the Cryptosporidium isolates. This study is the first to provide baseline information on Cryptosporidium contamination of Manila Bay where bivalves are commonly cultured. © 2017 Elsevier Ltd
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/87577
Appears in Collections:过去全球变化的重建
全球变化的国际研究计划

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作者单位: Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon City, Philippines

Recommended Citation:
Pagoso E.J.A.,Rivera W.L.. Cryptosporidium species from common edible bivalves in Manila Bay, Philippines[J]. Marine Pollution Bulletin,2017-01-01,119(1)
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