DOI: 10.1016/j.marpolbul.2017.04.036
Scopus记录号: 2-s2.0-85018784378
论文题名: Next-generation sequencing and culture-based techniques offer complementary insights into fungi and prokaryotes in beach sands
作者: Romão D. ; Staley C. ; Ferreira F. ; Rodrigues R. ; Sabino R. ; Veríssimo C. ; Wang P. ; Sadowsky M. ; Brandão J.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2017
卷: 119, 期: 1 起始页码: 351
结束页码: 358
语种: 英语
英文关键词: Beach sand
; Fungal community structure
; Monitoring
; Prokaryotic community structure
; Public health
Scopus关键词: Beaches
; Microorganisms
; Monitoring
; Public health
; Sand
; Social sciences
; Structural health monitoring
; Beach sand
; Community structures
; Culture-based methods
; Fecal indicators
; Fungal community
; Next-generation sequencing
; Pathogenic species
; Variable concentration
; Fungi
; bacterium
; beach
; community structure
; fungus
; pollution monitoring
; prokaryote
; public health
; relative abundance
; sand
; archaeon
; cell culture monitoring
; community structure
; dermatophyte
; fungal cell culture
; fungal community
; intermethod comparison
; next generation sequencing
; nonhuman
; Portugal
; prokaryote
; public health
; Purpureocillium
; seashore
; species
; bacterium
; environmental monitoring
; fungus
; genetics
; high throughput sequencing
; microbiology
; swimming
; Portugal
; Arthrodermataceae
; Bacteria (microorganisms)
; Fungi
; Prokaryota
; Bacteria
; Bathing Beaches
; Environmental Monitoring
; Fungi
; High-Throughput Nucleotide Sequencing
; Portugal
; Water Microbiology
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: A next-generation sequencing (NGS) approach, in conjunction with culture-based methods, was used to examine fungal and prokaryotic communities for the presence of potential pathogens in beach sands throughout Portugal. Culture-based fungal enumeration revealed low and variable concentrations of the species targeted (yeasts and dermatophytes), which were underrepresented in the community characterized by NGS targeting the ITS1 region. Conversely, NGS indicated that the potentially pathogenic species Purpureocillium liliacinum comprised nearly the entire fungal community. Culturable fecal indicator bacterial concentrations were low throughout the study and unrelated to communities characterized by NGS. Notably, the prokaryotic communities characterized revealed a considerable abundance of archaea. Results highlight differences in communities between methods in beach sand monitoring but indicate the techniques offer complementary insights. Thus, there is a need to leverage culture-based methods with NGS methods, using a toolbox approach, to determine appropriate targets and metrics for beach sand monitoring to adequately protect public health. © 2017 Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/87587
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: Water and Soils Unit – Department of Environmental Health, National Institute of Health Dr Ricardo Jorge, Av. Padre Cruz, Lisboa, Portugal; BioTechnology Institute, University of Minnesota, Saint Paul, MN, United States; Reference Unit for Parasitic and Fungal Infections – Department of Infectious Diseases, National Institute of Health Dr Ricardo Jorge, Av. Padre Cruz, Lisboa, Portugal; Department of Soil, Water, and Climate, University of Minnesota, Saint Paul, MN, United States
Recommended Citation:
Romão D.,Staley C.,Ferreira F.,et al. Next-generation sequencing and culture-based techniques offer complementary insights into fungi and prokaryotes in beach sands[J]. Marine Pollution Bulletin,2017-01-01,119(1)