Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要:
The potential risk of pharmaceuticals in the environment to top-predators is still largely unknown. In this study, we assessed the immunotoxic effects of ten pharmaceuticals individually and as mixtures on a harbor seal (Phoca vitulina) B lymphoma cell line. A significant reduction in lymphocyte transformation was observed following an exposure to 12,500�μg/L 17α-ethinyl estradiol and 25,000�μg/L naproxen. Exposure to 12,500�μg/L 17α-ethinyl estradiol decreased the percentage of cell in the G0/G1 phase of the cell cycle while increasing the percentage of cells in the S phase. Carbamazepine exposure increased the amount of cells in the G2/M phase. Binary mixtures showed synergistic effects in lymphocyte transformation, cell cycle and apoptosis assays. Concentrations inducing toxic effects in the cell line were similar to those affecting fish in previous studies. A reduction of functional activities of the immune system may lead to altered host resistance to pathogens in free-ranging pinnipeds. � 2017 Elsevier Ltd
INRS-Institut Armand-Frappier, Edifice 18, 531 blvd. des Prairies, Laval (QC), Canada; Department of Pathobiology and Veterinary Science, University of Connecticut, 61 North Eagleville Road, U-3089, Storrs, CT, United States
Recommended Citation:
Kleinert C.,Lacaze E.,Mounier M.,et al. Immunotoxic effects of single and combined pharmaceuticals exposure on a harbor seal (Phoca vitulina) B lymphoma cell line[J]. Marine Pollution Bulletin,2017-01-01,118(2018-01-02)