Beaches
; Inductively coupled plasma
; Mass spectrometry
; RNA
; 16S rRNA gene sequencing
; Enterococci
; Gram positive
; MALDI TOF MS
; Species identification
; Genes
; bacterium
; beach
; desorption
; gene
; ionization
; mass spectrometry
; protein
; RNA
; Article
; bacterium identification
; bacterium isolate
; Brazil
; controlled study
; Enterococcus
; Enterococcus casseliflavus
; Enterococcus durans
; Enterococcus faecium
; Enterococcus gallinarum
; Enterococcus hirae
; Enterococcus mundtii
; feasibility study
; gene sequence
; intermethod comparison
; marine environment
; matrix assisted laser desorption ionization time of flight mass spectrometry
; nonhuman
; recreation
; RNA gene
; seashore
; species identification
; classification
; Enterococcus
; genetics
; human
; isolation and purification
; matrix-assisted laser desorption-ionization mass spectrometry
; microbiology
; swimming
; Enterococcus
; Posibacteria
; RNA 16S
; sea water
; Bathing Beaches
; Brazil
; Enterococcus
; Humans
; RNA, Ribosomal, 16S
; Seawater
; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
; Water Microbiology
Scopus学科分类:
Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要:
MALDI-TOF Mass Spectrometry Biotyping has proven to be a reliable method for identifying bacteria at the species level based on the analysis of the ribosomal proteins mass fingerprint. We evaluate the usefulness of this method to identify Enterococcus species isolated from marine recreational water at Brazilian beaches. A total of 127 Enterococcus spp. isolates were identified to species level by bioM�rieux's API� 20 Strep and MALDI-TOF systems. The biochemical test identified 117/127 isolates (92%), whereas MALDI identified 100% of the isolates, with an agreement of 63% between the methods. The 16S rRNA gene sequencing of isolates with discrepant results showed that MALDI-TOF and API� correctly identified 74% and 11% of these isolates, respectively. This discrepancy probably relies on the bias of the API� has to identify clinical isolates. MALDI-TOF proved to be a feasible approach for identifying Enterococcus from environmental matrices increasing the rapidness and accuracy of results. � 2017 Elsevier Ltd
CETESB – Companhia Ambiental do Estado de S�o Paulo, Department of Environmental Analysis, S�o Paulo, SP, Brazil; UNIFESP – Universidade Federal de S�o Paulo, Department of Medicine, ALERTA Laboratory, S�o Paulo, SP, Brazil
Recommended Citation:
Christ A.P.G.,Ramos S.R.,Cay� R.,et al. Characterization of Enterococcus species isolated from marine recreational waters by MALDI-TOF MS and Rapid ID API� 20 Strep system[J]. Marine Pollution Bulletin,2017-01-01,118(2018-01-02)