DOI: 10.1016/j.marpolbul.2017.02.060
Scopus记录号: 2-s2.0-85014115518
论文题名: The constitutively active estrogen receptor (ER) binds and activates the promoter of the vitellogenin (Vtg) gene in the Sydney rock oyster, Saccostrea glomerata
作者: Tran T.K.A. ; MacFarlane G.R. ; Kong R.Y.C. ; O'Connor W.A. ; Yu R.M.K.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2017
卷: 118, 期: 2018-01-02 起始页码: 397
结束页码: 402
语种: 英语
英文关键词: Estrogen receptor
; Estrogen-responsive element
; Gene expression
; Molluscs
; Sydney rock oyster
; Vitellogenin
Scopus关键词: Animals
; Assays
; Bins
; Electrophoretic mobility
; Gene expression
; Genes
; Molluscs
; Shellfish
; Electrophoretic mobility shift assay
; Estrogen receptor
; Estrogen-responsive element
; Luciferase reporter assays
; Sydney rock oyster
; Transcriptional regulator
; Vitellogenin
; Well-established biomarkers
; Transcription
; estrogen
; estrogen receptor
; luciferase
; estrogen receptor
; vitellogenin
; biomarker
; bivalve
; enzyme
; estrogenic compound
; gene expression
; protein
; Article
; estrogen responsive element
; gel mobility shift assay
; gene
; gene expression
; gene mutation
; genetic transcription
; luciferase assay
; nonhuman
; promoter region
; receptor binding
; Saccostrea glomerata
; Vtg gene
; animal
; gene expression regulation
; genetics
; human
; metabolism
; oyster
; Animalia
; Mollusca
; Ostreidae
; Saccostrea glomerata
; Vertebrata
; Animals
; Gene Expression Regulation
; Humans
; Ostreidae
; Promoter Regions, Genetic
; Receptors, Estrogen
; Vitellogenins
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: Vitellogenin (Vtg) is a well-established biomarker of estrogenic exposure in aquatic animals. In vertebrates, Vtg gene transcription is controlled by the estrogen receptors (ERs). Although an ER ortholog is present in molluscs, its role as a transcriptional regulator remains elusive. Here, we tested the hypothesis that in the Sydney rock oyster, Saccostrea glomerata, the ER ortholog activates Vtg gene transcription through specific interaction with its promoter. Luciferase reporter assays indicated that sgER activated both a minimal promoter containing the consensus estrogen-responsive elements (EREs) and the sgVtg promoter in an estrogen-independent manner. The sgVtg promoter-luciferase activation was significantly reduced when any of three putative ERE half sites (½EREs) in the promoter were mutated. Electrophoretic mobility shift assay (EMSA) confirmed that sgER binds specifically to a 68-bp promoter sequence where these ½EREs reside. Overall, the results suggest that sgER is a constitutively active transcription factor that binds and activates the sgVtg promoter. © 2017 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/87669
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: School of Environmental and Life Sciences, The University of Newcastle, Callaghan, NSW, Australia; Department of Agriculture, Forestry and Fisheries, Vinh University, 182 Le Duan St., Vinh City, Nghe An, Viet Nam; Department of Biology and Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong; New South Wales Department of Primary Industries, Port Stephens Fisheries Institute, Taylors Beach, NSW, Australia
Recommended Citation:
Tran T.K.A.,MacFarlane G.R.,Kong R.Y.C.,et al. The constitutively active estrogen receptor (ER) binds and activates the promoter of the vitellogenin (Vtg) gene in the Sydney rock oyster, Saccostrea glomerata[J]. Marine Pollution Bulletin,2017-01-01,118(2018-01-02)