Cell death
; Cells
; Chemical compounds
; Cytology
; Endothelial cells
; Fish
; Oxygen
; Proteins
; Adaptive mechanism
; ASK1
; Biological functions
; Controlled environment
; HSP70
; Hypoxia-inducible factors
; Low oxygen tensions
; Total antioxidant capacities (TAC)
; Oxygen supply
; 4 hydroxynonenal
; apoptosis signal regulating kinase 1
; heat shock protein
; heat shock protein 70
; heme oxygenase 1
; hypoxia inducible factor 1alpha
; fish protein
; oxygen
; antioxidant
; apoptosis
; brain
; cell organelle
; gene expression
; homeostasis
; hypoxia
; protein
; survival
; animal cell
; Article
; cell isolation
; cell viability
; chemical parameters
; controlled study
; endothelium cell
; estuary
; in vitro study
; intracellular signaling
; nonhuman
; oxygen supply
; oxygen tension
; protein analysis
; protein expression
; protein function
; total antioxidant capacity
; transmission electron microscopy
; animal
; brain
; cytology
; endothelium cell
; metabolism
; oxidative stress
; Smegmamorpha
; Ennore Estuary
; India
; Kovalam Estuary
; Tamil Nadu
; Mugilidae
; Animals
; Brain
; Endothelial Cells
; Fish Proteins
; Oxidative Stress
; Oxygen
; Smegmamorpha
Scopus学科分类:
Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要:
The brain requires constant oxygen supply to perform its biological functions essential for survival. Because of low oxygen capacity and poor oxygen diffusibility of water, many fish species have evolved various adaptive mechanisms to cope with depleted oxygen. Endothelial cells (EC) are the primary components responsible for controlled environment of brain. Brain homeostasis largely depends on integrity of the EC. To elucidate their adaptive strategy, EC were isolated from the fish brain of Kovalam-control site and Ennore estuary-test/field hypoxic site and were subjected to low oxygen tension in laboratory. Cell viability, 4-hydroxynonenal (4HNE) and total antioxidant capacity (TAC) were analyzed to ascertain stress. Hypoxic insult, cytoprotective role of HSPs and apoptotic effect were analyzed by assessing hypoxia-inducible-factor-α (HIF1α), heat-shock-protein-70 (HSP70), heme-oxygenase 1 (HO-1), and apoptosis signal regulating kinase-1 (ASK1). This study evidenced that HSP70 and HO-1 are the key stress proteins, confer high tolerance to decreased oxygen tension mediated stress. � 2016 Elsevier Ltd