Biodegradation
; Bioremediation
; Biotechnology
; Carbon
; pH effects
; Soil pollution
; Contaminated soils
; Degrading bacteria
; Erythromycin A
; Isolation and characterization
; Ochrobactrum sp
; Propionaldehyde
; Sequence analysis
; Sole source of carbon
; Antibiotics
; 3 depyranosyloxy erythromycin A
; 6 deoxyerythronolide B
; 7,12 dihydroxy 6 deoxyerythronolide B
; DNA 16S
; drug metabolite
; erythromycin
; propionaldehyde
; unclassified drug
; erythromycin
; pollutant
; ribosome DNA
; RNA 16S
; biodegradation
; bioremediation
; concentration (composition)
; drug
; genetic analysis
; growth response
; microbial activity
; 16S rDNA gene
; Article
; bacterial growth
; bacterial metabolism
; bacterial strain
; bacterium identification
; bacterium isolation
; bioremediation
; carbon source
; China
; concentration response
; controlled study
; culture medium
; DNA sequence
; drug industry
; high performance liquid chromatography
; mass spectrometry
; microbial degradation
; nonhuman
; Ochrobactrum
; soil pollution
; temperature sensitivity
; analysis
; bioremediation
; chemical structure
; chemistry
; genetics
; isolation and purification
; metabolism
; microbiology
; Ochrobactrum
; pollutant
; temperature
; China
; Ochrobactrum sp.
; Biodegradation, Environmental
; China
; DNA, Ribosomal
; Environmental Pollutants
; Erythromycin
; Molecular Structure
; Ochrobactrum
; RNA, Ribosomal, 16S
; Soil Microbiology
; Temperature
Scopus学科分类:
Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要:
In this work, Erythromycin A(EA)- degrading bacteria was isolated from the contaminated soil obtained from a pharmaceutical factory in China. The isolate designated as strain WX-J1 was identified as Ochrobactrum sp. by sequence analysis of its 16S rDNA gene. It can grow in a medium containing EA as the sole source of carbon and its optimal growth pH and temperature were 6.5 and 32��C, respectively. Under these conditions, when the initial Erythromycin A concentration was 100�mg�L−�1, 97% of Erythromycin A has been degraded. HPLC–MS analyses indicated that Erythromycin A degradation produced intermediates contained the following three substances: 3-depyranosyloxy erythromycin A, 7,12-dyhydroxy-6-deoxyerythronolide B, 6-deoxyerythronolide B and propionaldehyde. Since Erythromycin A-degrading Ochrobactrum sp. strain rapidly degraded Erythromycin A, this strain might be useful for bioremediation purposes. � 2016 Elsevier Ltd