DOI: 10.1016/j.marpolbul.2017.06.049
Scopus记录号: 2-s2.0-85021429677
论文题名: Using a liver cell culture from Epinephelus coioides as a model to evaluate the nonylphenol-induced oxidative stress
作者: Derakhshesh N. ; Movahedinia A. ; Salamat N. ; Hashemitabar M. ; Bayati V.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2017
卷: 122, 期: 2018-01-02 起始页码: 243
结束页码: 252
语种: 英语
英文关键词: Epinephelus coioides
; Liver cell culture
; Nonylphenol
; Oxidative stress
Scopus关键词: Cell culture
; Cytology
; Oxidative stress
; Phenols
; Tissue culture
; Antioxidant systems
; Cellular metabolism
; Epinephelus coioides
; Liver cells
; Membrane disruption
; Nonylphenols
; Reactive oxygen species
; Short-term exposure
; Cells
; alanine aminotransferase
; alkaline phosphatase
; aspartate aminotransferase
; catalase
; collagenase
; glutathione peroxidase
; lactate dehydrogenase
; nonylphenol
; penicillin derivative
; phosphate buffered saline
; reactive oxygen metabolite
; streptomycin
; superoxide dismutase
; nonylphenol
; phenol derivative
; concentration (composition)
; fish
; modeling
; oxidative stress
; reactive oxygen species
; toxicity
; animal cell
; animal experiment
; animal tissue
; Article
; cell metabolism
; cell suspension
; cell viability
; controlled study
; enzyme activity
; enzyme analysis
; Epinephelus coioides
; IC50
; incubation temperature
; incubation time
; lipid peroxidation
; liver cell
; liver cell culture
; membrane damage
; monolayer culture
; MTT assay
; nonhuman
; oxidative stress
; tissue culture
; animal
; cytology
; drug effects
; enzymology
; fish
; liver
; toxicity
; water pollutant
; Bovinae
; Epinephelus coioides
; Animals
; Fishes
; Hepatocytes
; Liver
; Oxidative Stress
; Phenols
; Water Pollutants, Chemical
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: The present study aimed to use primary liver cell culture derived from the orange-spotted grouper, Epinephelus coioides, to assess the toxic effects of nonylphenol (NP) on the hepatocyte viability and the liver antioxidant system. E. coioides was selected due to its commercial importance. NP was used in this study because of its high potential of producing oxidative stress due to increased reactive oxygen species (ROS). A liver of E. coioides was digested with PBS containing 0.1% collagenase IV. The digested cells were moved to Leibovitz L-15 culture medium with 20% fetal bovine serum (FBS), 100 IU mL− 1 penicillin, 100 μg mL− 1 streptomycin. Aliquots of cell suspension were seeded as a monolayer into sterile 25 cm2 tissue culture flasks and incubated at 30 °C for 14 days. The medium, containing non-attached cells, was removed after 24 to 48 h and a new medium was added. The IC50 of 10− 4 mol L− 1 was determined for nonylphenol using MTT assay. Cells were then incubated with L-15 medium containing 10− 5, 2 × 10− 5, 3 × 10− 5 mol L− 1 of NP and samples were taken after 6, 12 and 24 h of incubation for analysis of LPO, SOD, CAT, GPx, LDH, AST, ALT, and ALP. Based on the results, the lowest concentration of NP was not markedly cytotoxic to primary hepatocytes and the cell sensitivity to NP increased dose-dependently. The activities of SOD, CAT and GPx decreased significantly, while activities of LPO, LDH, AST, ALT and ALP, increased significantly in a dose-related pattern in NP-treated cells. In conclusion, this study revealed that NP could induce the oxidative stress in cultivated hepatocytes of E. coioides during a short-term exposure. NP toxicity is mainly due to the induction of the reactive oxygen species (ROS), which lead to cell membrane disruption, damage of cellular metabolism, and interference with cellular macromolecules. © 2017 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/88094
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
There are no files associated with this item.
作者单位: Department of Marine Biology, Faculty of Marine Sciences, Khorramshahr University of Marine Science and Technology, Iran; Department of Anatomical Sciences, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Recommended Citation:
Derakhshesh N.,Movahedinia A.,Salamat N.,et al. Using a liver cell culture from Epinephelus coioides as a model to evaluate the nonylphenol-induced oxidative stress[J]. Marine Pollution Bulletin,2017-01-01,122(2018-01-02)