DOI: 10.1016/j.marpolbul.2017.06.027
Scopus记录号: 2-s2.0-85020638938
论文题名: Transcription of ribogenesis genes in fish gonads: Applications in the identification of stages of oogenesis and in environmental monitoring of intersex condition
作者: Rojo-Bartolomé I. ; Valencia A. ; Cancio I.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2017
卷: 121, 期: 2018-01-02 起始页码: 292
结束页码: 301
语种: 英语
英文关键词: 5S rRNA
; 5S/18S rRNA index
; Intersex severity
; Oocytes
; Oogenesis
; Xenoestrogenicity
Scopus关键词: Fish
; Genes
; Polymerase chain reaction
; Transcription
; 5S rRNA
; 5S/18S rRNA index
; Intersex severity
; Oocytes
; Oogenesis
; Xenoestrogenicity
; RNA
; RNA 18S
; RNA 5S
; cell organelle
; developmental stage
; environmental monitoring
; fish
; gene
; intersex
; polymerase chain reaction
; RNA
; adult
; animal tissue
; Article
; bioaccumulation
; biogenesis
; Chelon labrosus
; Chordata
; controlled study
; environmental monitoring
; female
; gene
; genetic transcription
; gonad development
; gtf3a gene
; intersex
; male
; nonhuman
; polymerase chain reaction
; quantitative analysis
; ribogenesis
; RNA synthesis
; ubtf1 gene
; animal
; disorder of sex development
; fish
; genetic transcription
; gonad
; metabolism
; oocyte development
; veterinary
; Chelon labrosus
; Mugilidae
; Animals
; Disorders of Sex Development
; Environmental Monitoring
; Female
; Fishes
; Gonads
; Male
; Oogenesis
; Transcription, Genetic
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: One of the best described effects of environmental xenoestrogens in fish is the generation of intersex gonads in males. Considering 5S rRNA a marker of the presence of oocytes, a 5S/18S rRNA index was calculated in 296 thicklip grey mullets (Chelon labrosus) from polluted environments. In addition, qPCR analysis of transcription factors gtf3a and ubtf1, related respectively to 5S and 18S rRNA synthesis, was conducted along female-oogenesis. 5S/18S rRNA index identified sex with a threshold value of 0.4521 separating males from females. Histological analysis identified 38 intersex individuals. Intersex severity and 5S/18S rRNA indexes were correlated. 5S/18S rRNA index identified ovarian developmental stage with high 5S rRNA levels during early oogenesis and 18S rRNA relative values increasing towards maturation. gtf3a and ubtf1 transcription levels followed the pattern of 5S rRNA accumulation. Thus, ribogenesis genes provide easy/quantitative methods to molecularly identify the sex, female gametogenic stage and intersex severity in mullets. © 2017 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/88147
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: CBET Research Group, Dept. of Zoology and Animal Cell Biology, Research Centre for Experimental Marine Biology and Biotechnology of Plentzia (PiE-UPV/EHU), University of the Basque Country (UPV/EHU), Areatza, z/g, Plentzia, Basque Country, Spain
Recommended Citation:
Rojo-Bartolomé I.,Valencia A.,Cancio I.. Transcription of ribogenesis genes in fish gonads: Applications in the identification of stages of oogenesis and in environmental monitoring of intersex condition[J]. Marine Pollution Bulletin,2017-01-01,121(2018-01-02)